TY - JOUR
T1 - 2-Arachidonoylglycerol impairs human cytotrophoblast cells syncytialization
T2 - influence of endocannabinoid signalling in placental development
AU - Costa, M. A.
AU - Keating, E.
AU - Fonseca, B. M.
AU - Teixeira, N. A.
AU - Correia-da-Silva, G.
N1 - Funding Information:
The authors thank Fundação para a Ciência e a Tecnologia (FCT) for the PhD grant of Mariana Costa ( SFRH/BD/70721/2010 ) and for the Post-doctoral grant of Bruno Fonseca ( SFRH/BPD/72958/2010 ). The authors also thank to the Department of Obstetrics and Gynaecology of S. João Hospital Center.
Publisher Copyright:
© 2014 Elsevier Ireland Ltd.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2015/1/5
Y1 - 2015/1/5
N2 - A balanced cytotrophoblast cell turnover is crucial for placental development and anomalies in this process associated with gestational diseases. The endocannabinoid system (ECS) has emerged as a new player in several biological processes. However, its influence during placental development is still unknown. We report here the expression of the endocannabinoid 2-arachidonoylglycerol (2-AG) main metabolic enzymes in human cytotrophoblasts and syncytiotrophoblast. We also showed that 2-AG induced a decrease in placental alkaline phosphatase activity, human chorionic gonadotropin secretion and Leptin mRNA levels. Moreover, 2-AG reduced glial cell missing 1 and syncytin-2 transcription and the number of nuclei in syncytium. These effects were mediated by cannabinoid receptors and may result from 2-AG inhibition of the cAMP/PKA signalling pathway. Our data suggest that 2-AG may interfere with the biochemical and morphological differentiation of human cytotrophoblasts, through a CB receptor-dependent mechanism, shedding light on a role for the ECS in placental development.
AB - A balanced cytotrophoblast cell turnover is crucial for placental development and anomalies in this process associated with gestational diseases. The endocannabinoid system (ECS) has emerged as a new player in several biological processes. However, its influence during placental development is still unknown. We report here the expression of the endocannabinoid 2-arachidonoylglycerol (2-AG) main metabolic enzymes in human cytotrophoblasts and syncytiotrophoblast. We also showed that 2-AG induced a decrease in placental alkaline phosphatase activity, human chorionic gonadotropin secretion and Leptin mRNA levels. Moreover, 2-AG reduced glial cell missing 1 and syncytin-2 transcription and the number of nuclei in syncytium. These effects were mediated by cannabinoid receptors and may result from 2-AG inhibition of the cAMP/PKA signalling pathway. Our data suggest that 2-AG may interfere with the biochemical and morphological differentiation of human cytotrophoblasts, through a CB receptor-dependent mechanism, shedding light on a role for the ECS in placental development.
KW - 2-arachinoylglycerol
KW - Endocannabinoid signalling
KW - Human cytotrophoblasts
KW - Placenta
KW - Syncytialization
UR - http://www.scopus.com/inward/record.url?scp=84912017615&partnerID=8YFLogxK
U2 - 10.1016/j.mce.2014.09.005
DO - 10.1016/j.mce.2014.09.005
M3 - Article
C2 - 25199616
AN - SCOPUS:84912017615
SN - 0303-7207
VL - 399
SP - 386
EP - 394
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
ER -