@article{7e6ab185cd064eeb994a92e34e97799d,
title = "A direct role for cohesin in gene regulation and ecdysone response in drosophila salivary glands",
abstract = "Background: Developmental abnormalities observed in Cornelia de Lange syndrome have been genetically linked to mutations in the cohesin machinery. These and other recent experimental findings have led to the suggestion that cohesin, in addition to its canonical function of mediating sister chromatid cohesion, might also be involved in regulating gene expression. Results: We report that cleavage of cohesin's kleisin subunit in postmitotic Drosophila salivary glands induces major changes in the transcript levels of many genes. Kinetic analyses of changes in transcript levels upon cohesin cleavage reveal that a subset of genes responds to cohesin cleavage within a few hours. In addition, cohesin binds to most of these loci, suggesting that cohesin is directly regulating their expression. Among these genes are several that are regulated by the steroid hormone ecdysone. Cytological visualization of transcription at selected ecdysone-responsive genes reveals that puffing at Eip74EF ceases within an hour or two of cohesin cleavage, long before any decline in ecdysone receptor could be detected at this locus. Conclusion: We conclude that cohesin regulates expression of a distinct set of genes, including those mediating the ecdysone response.",
author = "Andrea Pauli and {Van Bemmel}, {Joke G.} and Oliveira, {Raquel A.} and Takehiko Itoh and Katsuhiko Shirahige and {Van Steensel}, Bas and Kim Nasmyth",
note = "Funding Information: We thank B. Edgar, J. Mellor, and J. Mummery-Widmer (Knoblich laboratory) for reagents; Guillaume Filion for helping with the cohesin domain definition; B. Fischer (Fly-CHIP Cambridge) and Y. Katou for help with microarray analysis and ChIP-CHIP experiments, respectively; the central microarray facility from the Netherlands Cancer Institute for DamID-array hybridization; J. Metson, P. Guna, and W. Talhout for technical assistance; and all members of the B.v.S. and K.N. laboratories for discussions and comments on the manuscript. A.P. currently holds an EMBO Long-Term Fellowship. R.A.O. holds a postdoctoral fellowship from the Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia of Portugal. T.I. is supported by a Grant-in-Aid for Young Scientists (A) from the Japanese Society for the Promotion of Science (JSPS). Work in the laboratory of K.S. is supported by a grant of the Cell Innovation Program from Japan's Ministry of Education, Culture, Sports, Science and Technology and a Grant-in-Aid for Scientific Research (S) from the JSPS. Work in the laboratory of B.v.S. is supported by a Netherlands Genomics Initiative grant. Work in the laboratory of K.N. is supported by grants from the Medical Research Council and Wellcome Trust. ",
year = "2010",
month = oct,
day = "26",
doi = "10.1016/j.cub.2010.09.006",
language = "English",
volume = "20",
pages = "1787--1798",
journal = "Current Biology",
issn = "0960-9822",
publisher = "Cell Press",
number = "20",
}