A fluorescence-based optical fiber analyzer for catecholamine determination

Lurdes Silva; Kátia Duarte; Ana Cristina Freitas; Teresa S.L. Panteleitchouk; Teresa A. P. Rocha-Santos; Maria E. Pereira; Armando Costa Duarte

doi:10.1039/c2ay25076d

A fluorescence-based optical fiber analyzer for catecholamine determination

Lurdes Silva^*
, Kátia Duarte
, Ana Cristina Freitas
, Teresa S.L. Panteleitchouk
, Teresa A. P. Rocha-Santos
, Maria E. Pereira
, Armando Costa Duarte

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

An optical fibre (OF) analyzer for measuring catecholamines (dopamine, norepinephrine and epinephrine) in biological samples with induced fluorescence was developed. The analytical set-up included a chromatographic column for catecholamine separation and a fluorescence-based OF detection (FOF-analyzer). The detection limit of the FOF-analyser was found to be less than 0.9 pg mL -1. The proposed methodology showed an adequate analytical performance for the determination of the catecholamines in actual samples of human urine. The analytical performance of the FOF-analyzer for catecholamine determination was investigated against the high performance liquid chromatography-electrochemical detection (HPLC-ED) method. The FOF-analyzer showed lower detection limits and larger linear ranges for determination of dopamine (DA), norepinephrine (NE) and epinephrine (E) in comparison with HPLC-ED and other methodologies such as HPLC-fluorescence. These advantages combined with the compact design, small-scale instrumentation, and effective cost of analysis make this system an interesting alternative to the existing methodologies for the determination of catecholamines in clinical samples.

Original language	English
Pages (from-to)	2300-2306
Number of pages	7
Journal	Analytical Methods
Volume	4
Issue number	8
DOIs	https://doi.org/10.1039/c2ay25076d
Publication status	Published - Aug 2012
Externally published	Yes

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title = "A fluorescence-based optical fiber analyzer for catecholamine determination",

abstract = "An optical fibre (OF) analyzer for measuring catecholamines (dopamine, norepinephrine and epinephrine) in biological samples with induced fluorescence was developed. The analytical set-up included a chromatographic column for catecholamine separation and a fluorescence-based OF detection (FOF-analyzer). The detection limit of the FOF-analyser was found to be less than 0.9 pg mL -1. The proposed methodology showed an adequate analytical performance for the determination of the catecholamines in actual samples of human urine. The analytical performance of the FOF-analyzer for catecholamine determination was investigated against the high performance liquid chromatography-electrochemical detection (HPLC-ED) method. The FOF-analyzer showed lower detection limits and larger linear ranges for determination of dopamine (DA), norepinephrine (NE) and epinephrine (E) in comparison with HPLC-ED and other methodologies such as HPLC-fluorescence. These advantages combined with the compact design, small-scale instrumentation, and effective cost of analysis make this system an interesting alternative to the existing methodologies for the determination of catecholamines in clinical samples.",

author = "Lurdes Silva and K{\'a}tia Duarte and Freitas, \{Ana Cristina\} and Panteleitchouk, \{Teresa S.L.\} and Rocha-Santos, \{Teresa A. P.\} and Pereira, \{Maria E.\} and Duarte, \{Armando Costa\}",

year = "2012",

month = aug,

doi = "10.1039/c2ay25076d",

language = "English",

volume = "4",

pages = "2300--2306",

journal = "Analytical Methods",

issn = "1759-9660",

publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - A fluorescence-based optical fiber analyzer for catecholamine determination

AU - Silva, Lurdes

AU - Duarte, Kátia

AU - Freitas, Ana Cristina

AU - Panteleitchouk, Teresa S.L.

AU - Rocha-Santos, Teresa A. P.

AU - Pereira, Maria E.

AU - Duarte, Armando Costa

PY - 2012/8

Y1 - 2012/8

N2 - An optical fibre (OF) analyzer for measuring catecholamines (dopamine, norepinephrine and epinephrine) in biological samples with induced fluorescence was developed. The analytical set-up included a chromatographic column for catecholamine separation and a fluorescence-based OF detection (FOF-analyzer). The detection limit of the FOF-analyser was found to be less than 0.9 pg mL -1. The proposed methodology showed an adequate analytical performance for the determination of the catecholamines in actual samples of human urine. The analytical performance of the FOF-analyzer for catecholamine determination was investigated against the high performance liquid chromatography-electrochemical detection (HPLC-ED) method. The FOF-analyzer showed lower detection limits and larger linear ranges for determination of dopamine (DA), norepinephrine (NE) and epinephrine (E) in comparison with HPLC-ED and other methodologies such as HPLC-fluorescence. These advantages combined with the compact design, small-scale instrumentation, and effective cost of analysis make this system an interesting alternative to the existing methodologies for the determination of catecholamines in clinical samples.

AB - An optical fibre (OF) analyzer for measuring catecholamines (dopamine, norepinephrine and epinephrine) in biological samples with induced fluorescence was developed. The analytical set-up included a chromatographic column for catecholamine separation and a fluorescence-based OF detection (FOF-analyzer). The detection limit of the FOF-analyser was found to be less than 0.9 pg mL -1. The proposed methodology showed an adequate analytical performance for the determination of the catecholamines in actual samples of human urine. The analytical performance of the FOF-analyzer for catecholamine determination was investigated against the high performance liquid chromatography-electrochemical detection (HPLC-ED) method. The FOF-analyzer showed lower detection limits and larger linear ranges for determination of dopamine (DA), norepinephrine (NE) and epinephrine (E) in comparison with HPLC-ED and other methodologies such as HPLC-fluorescence. These advantages combined with the compact design, small-scale instrumentation, and effective cost of analysis make this system an interesting alternative to the existing methodologies for the determination of catecholamines in clinical samples.

UR - https://www.scopus.com/pages/publications/84864435054

U2 - 10.1039/c2ay25076d

DO - 10.1039/c2ay25076d

M3 - Article

AN - SCOPUS:84864435054

SN - 1759-9660

VL - 4

SP - 2300

EP - 2306

JO - Analytical Methods

JF - Analytical Methods

IS - 8

ER -

A fluorescence-based optical fiber analyzer for catecholamine determination

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