Abstract
Information on commercial aquaculture systems bacterial communities is scarce. The present study was performed in a turbot shallow raceway recirculating aquaculture system throughout 221 days after initial fish stocking. The dynamics of bacteria attached to a Moving Bed Biofilm Reactor and the distribution of the system culturable planktonic heterotrophic bacteria were assessed. Different levels of free heterotrophic bacteria were found over time: ≤2.6 × 10 4CFUmL -1 in incoming and ozonated water and ≥1.5 × 10 6CFUmL -1 in raceway and biofilter water. 16S rRNA gene sequencing of nine strains persistently isolated from the system affiliated with Bacteroidetes, α-Proteobacteria or γ-Proteobacteria. Polymerase chain reaction-denaturating gradient gel electrophoresis (PCR-DGGE) data showed temporal and spatial segregation of attached biofilter community. The first 32 days were the most important for bacterial establishment with higher culturable bacteria biomass loadings and higher community diversity. Multivariate canonical correspondence analysis of PCR-DGGE showed that the most common aquaculture water quality descriptors explained 70% of the DGGE pattern variability.
Original language | English |
---|---|
Pages (from-to) | 1308-1322 |
Number of pages | 15 |
Journal | Aquaculture Research |
Volume | 42 |
Issue number | 9 |
DOIs | |
Publication status | Published - Aug 2011 |
Keywords
- DGGE
- MBBR
- Microbial community
- Multivariate analysis
- Shallow raceway system