A molecular and multivariate approach to the microbial community of a commercial shallow raceway marine recirculation system operating with a moving bed biofilter

Ana Matos, Maria Teresa Borges*, Carla Peixe, Isabel Henriques, Carlos M. Pereira, Paula M. L. Castro

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)
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Abstract

Information on commercial aquaculture systems bacterial communities is scarce. The present study was performed in a turbot shallow raceway recirculating aquaculture system throughout 221 days after initial fish stocking. The dynamics of bacteria attached to a Moving Bed Biofilm Reactor and the distribution of the system culturable planktonic heterotrophic bacteria were assessed. Different levels of free heterotrophic bacteria were found over time: ≤2.6 × 10 4CFUmL -1 in incoming and ozonated water and ≥1.5 × 10 6CFUmL -1 in raceway and biofilter water. 16S rRNA gene sequencing of nine strains persistently isolated from the system affiliated with Bacteroidetes, α-Proteobacteria or γ-Proteobacteria. Polymerase chain reaction-denaturating gradient gel electrophoresis (PCR-DGGE) data showed temporal and spatial segregation of attached biofilter community. The first 32 days were the most important for bacterial establishment with higher culturable bacteria biomass loadings and higher community diversity. Multivariate canonical correspondence analysis of PCR-DGGE showed that the most common aquaculture water quality descriptors explained 70% of the DGGE pattern variability.
Original languageEnglish
Pages (from-to)1308-1322
Number of pages15
JournalAquaculture Research
Volume42
Issue number9
DOIs
Publication statusPublished - Aug 2011

Keywords

  • DGGE
  • MBBR
  • Microbial community
  • Multivariate analysis
  • Shallow raceway system

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