TY - JOUR
T1 - A transgenic flock house virus replicon reveals an RNAi independent antiviral mechanism acting in drosophila follicular somatic cells
AU - Martins, Nelson
AU - Lemoine, Aurélie
AU - Santiago, Estelle
AU - Paro, Simona
AU - Imler, Jean-Luc
AU - Meignin, Carine
N1 - Funding Information:
The authors thank J.T. Marques, E. Marois and two anonymous reviewers for critical comments that significantly improved the manuscript. Sequencing was performed by the IGBMC Microarray and Sequencing platform, a member of the ‘France Génomique’ consortium (ANR-10-INBS-0009). FACS sorting was done at the Flow Cytometry platform at Institut de Génétique et de Biologie Molécu-laire et Cellulaire (IGBMC, Strasbourg, France). This work was supported by the National Institutes of Health (PO1 AI070167), the Investissement d’Avenir Programs (ANR-10-LABX-0036 and ANR-11-EQPX-0022), Fondation ARC (PJA20151203110), Hoffmann Infinitus Program, CNRS, and INSERM. N.M. was supported by a Marie Skłodowska-Curie fellowship (MSCA/IF/2015/RESTRIVIR).
Publisher Copyright:
© 2019 Chen et al.
PY - 2019/2/1
Y1 - 2019/2/1
N2 - The small interfering RNA (siRNA) pathway is the main and best studied invertebrate antiviral response. Other poorly characterized protein based antiviral mechanisms also contribute to the control of viral replication in insects. In addition, it remains unclear whether tissue specific factors contribute to RNA and protein-based antiviral immunity mechanisms. In vivo screens to identify such factors are challenging and time consuming. In addition, the scored phenotype is usually limited to survival and/or viral load. Transgenic viral replicons are valuable tools to overcome these limitations and screen for novel antiviral factors. Here we describe transgenic Drosophila melanogaster lines encoding a Flock House Virus-derived replicon (FHV?B2eGFP), expressing GFP as a reporter of viral replication. This replicon is efficiently controlled by the siRNA pathway in most somatic tissues, with GFP fluorescence providing a reliable marker for the activity of antiviral RNAi. Interestingly, in follicular somatic cells (FSC) of ovaries, this replicon is still partially repressed in an siRNA independent manner. We did not detect replicon derived Piwi-interacting RNAs in FSCs and identified 31 differentially expressed genes between restrictive and permissive FSCs. Altogether, our results uncovered a yet unidentified RNAi-independent mechanism controlling FHV replication in FSCs of ovaries and validate the FHV?B2eGFP replicon as a tool to screen for novel tissue specific antiviral mechanisms.
AB - The small interfering RNA (siRNA) pathway is the main and best studied invertebrate antiviral response. Other poorly characterized protein based antiviral mechanisms also contribute to the control of viral replication in insects. In addition, it remains unclear whether tissue specific factors contribute to RNA and protein-based antiviral immunity mechanisms. In vivo screens to identify such factors are challenging and time consuming. In addition, the scored phenotype is usually limited to survival and/or viral load. Transgenic viral replicons are valuable tools to overcome these limitations and screen for novel antiviral factors. Here we describe transgenic Drosophila melanogaster lines encoding a Flock House Virus-derived replicon (FHV?B2eGFP), expressing GFP as a reporter of viral replication. This replicon is efficiently controlled by the siRNA pathway in most somatic tissues, with GFP fluorescence providing a reliable marker for the activity of antiviral RNAi. Interestingly, in follicular somatic cells (FSC) of ovaries, this replicon is still partially repressed in an siRNA independent manner. We did not detect replicon derived Piwi-interacting RNAs in FSCs and identified 31 differentially expressed genes between restrictive and permissive FSCs. Altogether, our results uncovered a yet unidentified RNAi-independent mechanism controlling FHV replication in FSCs of ovaries and validate the FHV?B2eGFP replicon as a tool to screen for novel tissue specific antiviral mechanisms.
KW - Antiviral immunity
KW - Drosophila melanogaster
KW - Follicular somatic cells
KW - Genetics of immunity
KW - Viral replicon
UR - http://www.scopus.com/inward/record.url?scp=85061231996&partnerID=8YFLogxK
U2 - 10.1534/g3.118.200872
DO - 10.1534/g3.118.200872
M3 - Article
C2 - 30530643
AN - SCOPUS:85061231996
SN - 2160-1836
VL - 9
SP - 403
EP - 412
JO - G3: Genes, Genomes, Genetics
JF - G3: Genes, Genomes, Genetics
IS - 2
ER -