TY - JOUR
T1 - Absorption kinetics of the main conjugated linoleic acid isomers in commercial-rich oil after oral administration in rats
AU - Rodríguez-Alcalá, Luís M.
AU - Ares, Irma
AU - Fontecha, Javier
AU - Martínez-Larrañaga, María Rosa
AU - Anadón, Arturo
AU - Martínez, María Aránzazu
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/9/6
Y1 - 2017/9/6
N2 - This study aimed to assess the oral absorption and plasma kinetics of two main isomers contained in commercial conjugated linoleic acid (CLA)-rich oil (Tonalin TG-80), rumenic acid (RA), and C18:2 trans-10, cis-12. The isomer plasma disposition after the single oral dose of 3000 mg of Tonalin TG-80/kg, containing 1200 mg/kg of each isomer, was studied in rats. The isomer plasma concentrations were determined by gas chromatography with flame ionization detection. The plasma kinetics showed rapid oral absorption of RA and C18:2 trans-10, cis-12 (t1/2a 0.34 ± 0.09 and 0.53 ± 0.01 h) and slow elimination (t1/2β 25.68 ± 3.29 and 18.12 ± 1.71 h); the maximal isomer plasma concentrations (Cmax) of 8.48 ± 0.98 and 7.67 ± 0.80 μg mL-1, respectively, were estimated at 2.08 ± 0.14 and 2.26 ± 0.11 h. Our results from a preclinical kinetic study in rats help to design future studies in humans for evaluating the CLA isomer dose-response.
AB - This study aimed to assess the oral absorption and plasma kinetics of two main isomers contained in commercial conjugated linoleic acid (CLA)-rich oil (Tonalin TG-80), rumenic acid (RA), and C18:2 trans-10, cis-12. The isomer plasma disposition after the single oral dose of 3000 mg of Tonalin TG-80/kg, containing 1200 mg/kg of each isomer, was studied in rats. The isomer plasma concentrations were determined by gas chromatography with flame ionization detection. The plasma kinetics showed rapid oral absorption of RA and C18:2 trans-10, cis-12 (t1/2a 0.34 ± 0.09 and 0.53 ± 0.01 h) and slow elimination (t1/2β 25.68 ± 3.29 and 18.12 ± 1.71 h); the maximal isomer plasma concentrations (Cmax) of 8.48 ± 0.98 and 7.67 ± 0.80 μg mL-1, respectively, were estimated at 2.08 ± 0.14 and 2.26 ± 0.11 h. Our results from a preclinical kinetic study in rats help to design future studies in humans for evaluating the CLA isomer dose-response.
KW - C18:2 trans 10
KW - Cis 12
KW - CLA isomers
KW - Oral absorption
KW - Plasma disposition
KW - RA
KW - Rats
UR - http://www.scopus.com/inward/record.url?scp=85028923590&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.7b02143
DO - 10.1021/acs.jafc.7b02143
M3 - Article
C2 - 28789519
AN - SCOPUS:85028923590
SN - 0021-8561
VL - 65
SP - 7680
EP - 7686
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 35
ER -