ACE-inhibitory activity assay: IC50

Ezequiel Coscueta; Maria Manuela Pintado

doi:10.17504/protocols.io.q26g74q5kgwz/v1

ACE-inhibitory activity assay: IC50

Ezequiel Coscueta^*
, Maria Manuela Pintado

^*Corresponding author for this work

Research output: Other contribution

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Abstract

This protocol describes the procedure for the determination of the IC50 in inhibition on angiotensin-I converting enzyme (ACE) activity. ACE is also known as peptidyl dipeptidase A because it removes C-terminal dipeptides from a wide variety of peptide substrates. In the assay described here, the chosen substrate is the intramolecularly quenched fluorescent tripeptide o-aminobenzoylglycyl-p-nitrophenylalanylproline (Abz–Gly–Phe(NO2)–Pro). Hydrolysis of this substrate by the action of ACE generates the fluorescent product o-aminobenzoylglycine (Abz–Gly).

Original language	English
Type	Protocol
Publisher	Protocols.io
Number of pages	3
DOIs	https://doi.org/10.17504/protocols.io.q26g74q5kgwz/v1
Publication status	Published - 17 Aug 2022

Keywords

ACE
Angiotensin-converting enzyme
Antihypertensive
IC50
Bioactivity
Angiotensin-l converting enzyme
Angiotensin
Inhibitory activity assay
Ic50 in inhibition
Nitrophenylalanylproline
Enzyme
Terminal dipeptide
Peptide substrate
Action of ace
Converting enzyme
Assay
Ace

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10.17504/protocols.io.q26g74q5kgwz/v1Licence: CC BY

61480817_stepsFinal published version, 582 KBLicence: CC BY
61480817_materialsFinal published version, 590 KBLicence: CC BY

http://hdl.handle.net/10400.14/58056Licence: CC BY

1 Article

Enzymatic soy protein hydrolysis: a tool for biofunctional food ingredient production
Coscueta, E. R., Campos, D. A., Osório, H., Nerli, B. B. & Pintado, M., 30 Mar 2019, In: Food Chemistry: X. 1, 7 p., 100006.
Research output: Contribution to journal › Article › peer-review

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@misc{60d09b679ba44b2d879ee92b4a11f1a9,

title = "ACE-inhibitory activity assay: IC50",

abstract = "This protocol describes the procedure for the determination of the IC50 in inhibition on angiotensin-I converting enzyme (ACE) activity. ACE is also known as peptidyl dipeptidase A because it removes C-terminal dipeptides from a wide variety of peptide substrates. In the assay described here, the chosen substrate is the intramolecularly quenched fluorescent tripeptide o-aminobenzoylglycyl-p-nitrophenylalanylproline (Abz–Gly–Phe(NO2)–Pro). Hydrolysis of this substrate by the action of ACE generates the fluorescent product o-aminobenzoylglycine (Abz–Gly). ",

keywords = "ACE, Angiotensin-converting enzyme, Antihypertensive, IC50, Bioactivity, Angiotensin-l converting enzyme, Angiotensin, Inhibitory activity assay, Ic50 in inhibition, Nitrophenylalanylproline, Enzyme, Terminal dipeptide, Peptide substrate, Action of ace, Converting enzyme, Assay, Ace",

author = "Ezequiel Coscueta and Pintado, \{Maria Manuela\}",

year = "2022",

month = aug,

day = "17",

doi = "10.17504/protocols.io.q26g74q5kgwz/v1",

language = "English",

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T2 - IC50

AU - Coscueta, Ezequiel

AU - Pintado, Maria Manuela

PY - 2022/8/17

Y1 - 2022/8/17

N2 - This protocol describes the procedure for the determination of the IC50 in inhibition on angiotensin-I converting enzyme (ACE) activity. ACE is also known as peptidyl dipeptidase A because it removes C-terminal dipeptides from a wide variety of peptide substrates. In the assay described here, the chosen substrate is the intramolecularly quenched fluorescent tripeptide o-aminobenzoylglycyl-p-nitrophenylalanylproline (Abz–Gly–Phe(NO2)–Pro). Hydrolysis of this substrate by the action of ACE generates the fluorescent product o-aminobenzoylglycine (Abz–Gly).

AB - This protocol describes the procedure for the determination of the IC50 in inhibition on angiotensin-I converting enzyme (ACE) activity. ACE is also known as peptidyl dipeptidase A because it removes C-terminal dipeptides from a wide variety of peptide substrates. In the assay described here, the chosen substrate is the intramolecularly quenched fluorescent tripeptide o-aminobenzoylglycyl-p-nitrophenylalanylproline (Abz–Gly–Phe(NO2)–Pro). Hydrolysis of this substrate by the action of ACE generates the fluorescent product o-aminobenzoylglycine (Abz–Gly).

KW - ACE

KW - Angiotensin-converting enzyme

KW - Antihypertensive

KW - IC50

KW - Bioactivity

KW - Angiotensin-l converting enzyme

KW - Angiotensin

KW - Inhibitory activity assay

KW - Ic50 in inhibition

KW - Nitrophenylalanylproline

KW - Enzyme

KW - Terminal dipeptide

KW - Peptide substrate

KW - Action of ace

KW - Converting enzyme

KW - Assay

KW - Ace

U2 - 10.17504/protocols.io.q26g74q5kgwz/v1

DO - 10.17504/protocols.io.q26g74q5kgwz/v1

M3 - Other contribution

PB - Protocols.io

ER -

ACE-inhibitory activity assay: IC50

Abstract

Keywords

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Research output

Enzymatic soy protein hydrolysis: a tool for biofunctional food ingredient production

Cite this