TY - JOUR
T1 - Acinetobacter portensis sp. nov. and acinetobacter guerrae sp. nov., isolated from raw meat
AU - Carvalheira, Ana
AU - Gonzales-Siles, Lucia
AU - Salvà-Serra, Francisco
AU - Lindgren, Åsa
AU - Svensson-Stadler, Liselott
AU - Thorell, Kaisa
AU - Piñeiro-Iglesias, Beatriz
AU - Karlsson, Roger
AU - Silva, Joana
AU - Teixeira, Paula
AU - Moore, Edward R. B.
N1 - Funding Information:
This work was supported through project ‘Biological tools for adding and defending value in key agro-food chains (bio – n2 – value)', no. NORTE-01–0145-FEDER-000030, funded by Fundo Europeu de Desenvolvimento Regional (FEDER), under Programa Operacional Regional do Norte - Norte2020. We thank the scientific collaboration under the Fundação para a Ciência e a Tecnologia (FCT) project UID/ Multi/50016/2019. Financial support for A.C. and J.S. was provided by FCT through PhD fellowship SFRH/BD/72951/2010 and postdoctoral fellowship SFRH/BPD/35392/2007, respectively. L.G.-S., F.S.-S. and E.R.B.M. acknowledge support from the European Commission (seventh Framework Programme: ‘Tailored-Treatment’, project no. 602860), the Swedish Västra Götaland Region (project nos. ALFGBG-437221 and ALFGBG-720761; Lab Medicine project no. 51060–6268) and the University of Gothenburg Global Challenges project and Centre for Antibiotic Resistance Research (CARe) (project no. 205314021). F.S.S. was supported by a stipend for Basic and Advanced Research from the CCUG. The Culture Collection University of Gothenburg (CCUG) is supported by the Department of Clinical Microbiology, Sahlgrenska University Hospital and the Sahlgrenska Academy of the University of Gothenburg.
Funding Information:
The authors acknowledge the expertise of Kent Molin (CCUG) for the analysis of cellular fatty acids, the technical assistance of Timur Tunovic for MALDI-TOF MS analyses, as well as all the CCUG staff for technical assistance with cultivation and characterization of the strains. The authors thank Lars Engstrand and SciLifeLab for their support in the genome sequence determinations.
Publisher Copyright:
© 2020 The Authors.
PY - 2020/8/1
Y1 - 2020/8/1
N2 - The taxonomic status of six strains of Acinetobacter obtained from meat samples, collected from supermarkets in Porto, Portugal, was investigated using polyphasic analysis. Partial rpoB sequence similarities lower than 95 % to other Acinetobacter species with validly published names led to the hypothesis that these strains represented novel species. This was confirmed based on comparative multilocus sequence analysis, which included the gyrB, recA and 16S rRNA genes, revealing that these strains represented two coherent lineages that were distinct from each other and from all known species. The names Acinetobacter portensis sp. nov. (comprising four strains) and Acinetobacter guerrae sp. nov. (comprising two strains) are proposed for these novel species. The species status of these two groups was confirmed by low (below 95 %) whole-genome sequence average nucleotide identity values and low (below 70 %) digital DNA-DNA hybridization similarities between the whole-genome sequences of the proposed type strains of each novel species and the representatives of the known Acinetobacter species. Phylogenomic treeing from core genome analysis supported these results. The coherence of each new species lineage was supported by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry differentiation of the species at the protein level, by cellular fatty acid profiles, and by unique and differential combinations of metabolic and physiological properties shared by each novel species. The type strain of A. portensis sp. nov. is AC 877T (=CCUG 68672T=CCM 8789T) and the type strain of A. guerrae sp. nov. is AC 1271T (=CCUG 68674T=CCM 8791T).
AB - The taxonomic status of six strains of Acinetobacter obtained from meat samples, collected from supermarkets in Porto, Portugal, was investigated using polyphasic analysis. Partial rpoB sequence similarities lower than 95 % to other Acinetobacter species with validly published names led to the hypothesis that these strains represented novel species. This was confirmed based on comparative multilocus sequence analysis, which included the gyrB, recA and 16S rRNA genes, revealing that these strains represented two coherent lineages that were distinct from each other and from all known species. The names Acinetobacter portensis sp. nov. (comprising four strains) and Acinetobacter guerrae sp. nov. (comprising two strains) are proposed for these novel species. The species status of these two groups was confirmed by low (below 95 %) whole-genome sequence average nucleotide identity values and low (below 70 %) digital DNA-DNA hybridization similarities between the whole-genome sequences of the proposed type strains of each novel species and the representatives of the known Acinetobacter species. Phylogenomic treeing from core genome analysis supported these results. The coherence of each new species lineage was supported by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry differentiation of the species at the protein level, by cellular fatty acid profiles, and by unique and differential combinations of metabolic and physiological properties shared by each novel species. The type strain of A. portensis sp. nov. is AC 877T (=CCUG 68672T=CCM 8789T) and the type strain of A. guerrae sp. nov. is AC 1271T (=CCUG 68674T=CCM 8791T).
KW - Acinetobacter
KW - Core genome
KW - MLSA
KW - Novel species in meat
KW - Phylogenomics
KW - Whole-genome sequence
UR - http://www.scopus.com/inward/record.url?scp=85090070261&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.004311
DO - 10.1099/ijsem.0.004311
M3 - Article
C2 - 32618559
AN - SCOPUS:85090070261
SN - 1466-5026
VL - 70
SP - 4544
EP - 4554
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 8
ER -