Acrylic bone cement induces the production of free radicals by cultured human fibroblasts

Fernando Martins Vale, Matilde Castro, Jacinto Monteiro, Frederico S. Couto, Rui Pinto, J. M.Gião Toscano Rico

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)

Abstract

Arthroplasty with poly(methyl methacrylate) (PMMA) bone cement induces late loosening phenomena that compromise the prosthetic stability. As free radicals are inflammatory mediators and cytotoxic, it seemed useful to investigate whether PMMA induces the liberation of free radicals and/or cytotoxicity. The effect of PMMA interaction on cultured human fibroblasts was accessed by the cell viability test (MTT), and by the measurement of lipoperoxides in the incubation medium. The incubation with the medium exposed to PMMA induced a significant reduction in the viability and a significant increase in lipoperoxide liberation (vs control). These data suggest that PMMA is cytotoxic. This effect seems to be mediated by lipoperoxide and possibly by other free radicals, and may explain the peri-implant loosening phenomena that compromise the prosthetic stability.
Original languageEnglish
Pages (from-to)1133-1135
Number of pages3
JournalBiomaterials
Volume18
Issue number16
DOIs
Publication statusPublished - Aug 1997
Externally publishedYes

Keywords

  • Cell viability
  • Free radicals
  • Lipoperoxides
  • Poly(methyl methacrylate) bone cement

Fingerprint

Dive into the research topics of 'Acrylic bone cement induces the production of free radicals by cultured human fibroblasts'. Together they form a unique fingerprint.

Cite this