Acrylic bone cement induces the production of free radicals by cultured human fibroblasts

Fernando Martins Vale; Matilde Castro; Jacinto Monteiro; Frederico S. Couto; Rui Pinto; J. M.Gião Toscano Rico

doi:10.1016/S0142-9612(97)00043-4

Acrylic bone cement induces the production of free radicals by cultured human fibroblasts

Fernando Martins Vale, Matilde Castro, Jacinto Monteiro, Frederico S. Couto, Rui Pinto, J. M.Gião Toscano Rico

Research output: Contribution to journal › Article › peer-review

59 Citations (Scopus)

Abstract

Arthroplasty with poly(methyl methacrylate) (PMMA) bone cement induces late loosening phenomena that compromise the prosthetic stability. As free radicals are inflammatory mediators and cytotoxic, it seemed useful to investigate whether PMMA induces the liberation of free radicals and/or cytotoxicity. The effect of PMMA interaction on cultured human fibroblasts was accessed by the cell viability test (MTT), and by the measurement of lipoperoxides in the incubation medium. The incubation with the medium exposed to PMMA induced a significant reduction in the viability and a significant increase in lipoperoxide liberation (vs control). These data suggest that PMMA is cytotoxic. This effect seems to be mediated by lipoperoxide and possibly by other free radicals, and may explain the peri-implant loosening phenomena that compromise the prosthetic stability.

Original language	English
Pages (from-to)	1133-1135
Number of pages	3
Journal	Biomaterials
Volume	18
Issue number	16
DOIs	https://doi.org/10.1016/S0142-9612(97)00043-4
Publication status	Published - Aug 1997
Externally published	Yes

Keywords

Cell viability
Free radicals
Lipoperoxides
Poly(methyl methacrylate) bone cement

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10.1016/S0142-9612(97)00043-4

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title = "Acrylic bone cement induces the production of free radicals by cultured human fibroblasts",

abstract = "Arthroplasty with poly(methyl methacrylate) (PMMA) bone cement induces late loosening phenomena that compromise the prosthetic stability. As free radicals are inflammatory mediators and cytotoxic, it seemed useful to investigate whether PMMA induces the liberation of free radicals and/or cytotoxicity. The effect of PMMA interaction on cultured human fibroblasts was accessed by the cell viability test (MTT), and by the measurement of lipoperoxides in the incubation medium. The incubation with the medium exposed to PMMA induced a significant reduction in the viability and a significant increase in lipoperoxide liberation (vs control). These data suggest that PMMA is cytotoxic. This effect seems to be mediated by lipoperoxide and possibly by other free radicals, and may explain the peri-implant loosening phenomena that compromise the prosthetic stability.",

keywords = "Cell viability, Free radicals, Lipoperoxides, Poly(methyl methacrylate) bone cement",

author = "Vale, {Fernando Martins} and Matilde Castro and Jacinto Monteiro and Couto, {Frederico S.} and Rui Pinto and Rico, {J. M.Gi{\~a}o Toscano}",

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T1 - Acrylic bone cement induces the production of free radicals by cultured human fibroblasts

AU - Vale, Fernando Martins

AU - Castro, Matilde

AU - Monteiro, Jacinto

AU - Couto, Frederico S.

AU - Pinto, Rui

AU - Rico, J. M.Gião Toscano

PY - 1997/8

Y1 - 1997/8

N2 - Arthroplasty with poly(methyl methacrylate) (PMMA) bone cement induces late loosening phenomena that compromise the prosthetic stability. As free radicals are inflammatory mediators and cytotoxic, it seemed useful to investigate whether PMMA induces the liberation of free radicals and/or cytotoxicity. The effect of PMMA interaction on cultured human fibroblasts was accessed by the cell viability test (MTT), and by the measurement of lipoperoxides in the incubation medium. The incubation with the medium exposed to PMMA induced a significant reduction in the viability and a significant increase in lipoperoxide liberation (vs control). These data suggest that PMMA is cytotoxic. This effect seems to be mediated by lipoperoxide and possibly by other free radicals, and may explain the peri-implant loosening phenomena that compromise the prosthetic stability.

AB - Arthroplasty with poly(methyl methacrylate) (PMMA) bone cement induces late loosening phenomena that compromise the prosthetic stability. As free radicals are inflammatory mediators and cytotoxic, it seemed useful to investigate whether PMMA induces the liberation of free radicals and/or cytotoxicity. The effect of PMMA interaction on cultured human fibroblasts was accessed by the cell viability test (MTT), and by the measurement of lipoperoxides in the incubation medium. The incubation with the medium exposed to PMMA induced a significant reduction in the viability and a significant increase in lipoperoxide liberation (vs control). These data suggest that PMMA is cytotoxic. This effect seems to be mediated by lipoperoxide and possibly by other free radicals, and may explain the peri-implant loosening phenomena that compromise the prosthetic stability.

KW - Cell viability

KW - Free radicals

KW - Lipoperoxides

KW - Poly(methyl methacrylate) bone cement

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VL - 18

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JO - Biomaterials

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Acrylic bone cement induces the production of free radicals by cultured human fibroblasts

Abstract

Keywords

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