Anthocyanins: quantification by tandem mass spectrometry

Cindy Dias, Manuela Pintado, Clara Sousa*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Mass spectrometry is a high throughput technique widely used for metabolic fingerprinting of plant material. Among the diverse plant metabolites, pigments such as anthocyanins play a determinant role in plant defence mechanisms, protecting them from biotic and abiotic stresses. Anthocyanins are phenolic water-soluble glycosides or acyl-glycosides of anthocyanidins which could be accurately detected and quantified through mass spectrometry. This chapter describes how to extract anthocyanins from higher plant materials and quantify them through a liquid chromatography-mass spectrometry (LC-MS) based method.

Original languageEnglish
Title of host publicationROS Signaling in Plants
EditorsFrancisco J. Corpas, José M. Palma
PublisherHumana Press
Pages153-159
Number of pages7
Volume2798
Edition1
ISBN (Electronic)9781071638262
ISBN (Print)9781071638255, 9781071638286
DOIs
Publication statusPublished - 2024

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745

Keywords

  • Anthocyanins
  • HPLC
  • Mass spectrometry (MS)
  • Metabolites
  • Pigments
  • Plant
  • Solid phase extraction (SPE)

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