Anti-Acanthamoeba activity of a semi-synthetic mangostin derivative and its ability in removal of Acanthamoeba triangularis WU19001 on contact lens

Julalak Chuprom, Suthinee Sangkanu, Watcharapong Mitsuwan, Rachasak Boonhok, Wilawan Mahabusarakam, L. Ravithej Singh, Ekachai Dumkliang, Kritamorn Jitrangsri, Alok K. Paul, Sirirat Surinkaew, Polrat Wilairatana, Maria de Lourdes Pereira, Mohammed Rahmatullah, Christophe Wiart, Sonia Marlene Rodrigues Oliveira, Veeranoot Nissapatorn*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

Garcinia mangostana L., also known as the mangosteen tree, is a native medicinal plant in Southeast Asia having a wide variety of pharmacologically active compounds, including xanthonoid mangostin. In this study, we examined the pharmacological activities of the selected semi-synthetic mangostin derivative, namely, amoebicidal activity, encystation inhibition, excystation activity, and removal capacity of adhesive Acanthamoeba from the surface of contact lens (CL). Among the three derivatives, C1 exhibited promising anti-Acanthamoeba activity against Acanthamoeba triangularis WU19001 trophozoites and cysts. SEM images displayed morphological changes in Acanthamoeba trophozoites, including the loss of acanthopodia, pore formation in the cell membrane, and membrane damage. In addition, the treated cyst was shrunken and adopted an irregular flat cyst shape. Under a fluorescence microscope, acridine orange and propidium iodide (AO/PI) staining revealed C1 induced condensation of cytoplasm and chromatin with the loss of cell volume in the treated trophozoites, while calcofluor white staining demonstrated the leakage of cell wall in treated cysts, leading to cell death. Interestingly, at the concentration ranges in which C1 showed the anti-Acanthamoeba effects (IC50 values ranging from 0.035–0.056 mg/mL), they were not toxic to Vero cells. C1 displayed the highest inhibitory effect on A. triangularis encystation at 1/16×MIC value (0.004 mg/mL). While C1 demonstrated the excystation activity at 1/128×MIC value with a high rate of 89.47%. Furthermore, C1 exhibited the removal capacity of adhesive Acanthamoeba from the surface of CL comparable with commercial multipurpose solutions (MPSs). Based on the results obtained, C1 may be a promising lead agent to develop a therapeutic for the treatment of Acanthamoeba infections and disinfectant solutions for CL.
Original languageEnglish
Article numbere14468
Number of pages27
JournalPeerJ
Volume10
DOIs
Publication statusPublished - 9 Dec 2022
Externally publishedYes

Keywords

  • Mangostin
  • Garcinia mangostana
  • Acanthamoeba triangularis
  • Anti-Acanthamoeba activity
  • Amoebicidal activity
  • Contact lens
  • Semi-synthetic derivative

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