TY - JOUR
T1 - Anti-inflammatory and antioxidant activity improvement of lycopene from guava on nanoemulsifying system
AU - Amorim, Adriany
AU - Souza, Jéssica
AU - Oliveira, Ana
AU - Santos, Raimunda
AU - Vasconcelos, Andreanne
AU - Souza, Luan
AU - Araujo, Thiago
AU - Cabral, Wanessa
AU - Silva, Maria
AU - Mafud, Ana
AU - Yvonne, Mascarenhas
AU - Medeiros, Jand-Venes
AU - Saraiva, Jaco
AU - Muehlmann, Luis
AU - Kuckelhaus, Selma
AU - Pintado, Manuela
AU - Leite, José R.
N1 - Funding Information:
A. Amorim is grateful to CAPES by PDSE process n° 99999.004236/2014-09 in the Portuguese Catholic University (UCP) and to the Center for Biotechnology and Fine Chemistry (CBQF). BIOTEC is grateful to Phytobios LTDA and Centroflora Group, for the reagents kindly supplied during the development of this work.
Funding Information:
A. Oliveira is grateful to the Foundation to Science and Technology (FCT) for the PhD grant SFRH/BD/75041/2010. A. Mafud is grateful to FAPESP (grant 2014/02282-6). Y. Mascarenhas was supported by CNPq (Grant 165177/2014-4). A. G. Vasconcelos is grateful to CAPES for grant n° 1713871. This work was supported by National Funds from FCT–Fundação para a Ciência e a Tecnologia through project PEst-OE/EQB/LA0016/2013. A. Amorim is grateful to CAPES by PDSE process n° 99999.004236/2014-09 in the Portuguese Catholic University (UCP) and to the Center for Biotechnology and Fine Chemistry (CBQF). BIOTEC is grateful to Phytobios LTDA and Centroflora Group, for the reagents kindly supplied during the development of this work.
Publisher Copyright:
© 2020 Taylor & Francis Group, LLC.
PY - 2020
Y1 - 2020
N2 - The nanoemulsion system can improve stability to antioxidant molecules when compared to the isolated molecule. Therefore, this study aimed to establish conditions on thermal stability, chemical characterization, antioxidant and anti-inflammatory activity of lycopene nanoemulsion. Different chemical assays were used to characterize the lycopene nanoemulsion (LN) and the purified lycopene from guava (LPG) as HPLC, UV-Vis, DLS, and ABTS to analyze the antioxidant activity of lycopene nanoemulsion. To test its thermal stability as an antioxidant in nanoemulsion it was used the thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) techniques. The carrageenan-induced paw edema model in Swiss mice and histopathological analysis were used to evaluate anti-inflammatory activity. Emulsion with a mean droplet size of z-Average diameter 167.1 d.nm was produced. UV-vis detected λ max = 447.1, 473.7, and 505.3 nm to lycopene from guava and HPLC-DAD analysis by C 30 at 472 nm identified the presence of lycopene in the nanoemulsion. This lycopene nanoemulsion presented thermal stability at room temperature, as well as on concentration when stored for one year. Also significantly inhibited (p < 0.05) the edema formation, with maximum inhibitory effect at 50 mg/kg by oral and intraperitoneal route and reduced neutrophil migration in paw tissue. The lycopene nanoemulsion can be a potential candidate for investigation and therapy of various diseases, including inflammation because this lycopene nanoemulsion presented therapeutics application for inflammatory diseases, and has also potential to be used as ointment and/or cream additive for topic applications.
AB - The nanoemulsion system can improve stability to antioxidant molecules when compared to the isolated molecule. Therefore, this study aimed to establish conditions on thermal stability, chemical characterization, antioxidant and anti-inflammatory activity of lycopene nanoemulsion. Different chemical assays were used to characterize the lycopene nanoemulsion (LN) and the purified lycopene from guava (LPG) as HPLC, UV-Vis, DLS, and ABTS to analyze the antioxidant activity of lycopene nanoemulsion. To test its thermal stability as an antioxidant in nanoemulsion it was used the thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) techniques. The carrageenan-induced paw edema model in Swiss mice and histopathological analysis were used to evaluate anti-inflammatory activity. Emulsion with a mean droplet size of z-Average diameter 167.1 d.nm was produced. UV-vis detected λ max = 447.1, 473.7, and 505.3 nm to lycopene from guava and HPLC-DAD analysis by C 30 at 472 nm identified the presence of lycopene in the nanoemulsion. This lycopene nanoemulsion presented thermal stability at room temperature, as well as on concentration when stored for one year. Also significantly inhibited (p < 0.05) the edema formation, with maximum inhibitory effect at 50 mg/kg by oral and intraperitoneal route and reduced neutrophil migration in paw tissue. The lycopene nanoemulsion can be a potential candidate for investigation and therapy of various diseases, including inflammation because this lycopene nanoemulsion presented therapeutics application for inflammatory diseases, and has also potential to be used as ointment and/or cream additive for topic applications.
KW - Anti-inflammatory
KW - lycopene
KW - Nanoemulsion
KW - Thermal behavior
UR - http://www.scopus.com/inward/record.url?scp=85080975127&partnerID=8YFLogxK
U2 - 10.1080/01932691.2020.1728300
DO - 10.1080/01932691.2020.1728300
M3 - Article
SN - 0193-2691
VL - 42
SP - 760
EP - 770
JO - Journal of Dispersion Science and Technology
JF - Journal of Dispersion Science and Technology
IS - 5
ER -