TY - JOUR
T1 - Anti-viral photodynamic inactivation of T4-like bacteriophage as a mammalian virus model in blood
AU - Santos, Patrícia
AU - Gomes, Ana T. P. C.
AU - Lourenço, Leandro M. O.
AU - Faustino, Maria A. F.
AU - Neves, Maria G. P. M. S.
AU - Almeida, Adelaide
N1 - Funding Information:
Thanks are due to the University of Aveiro and FCT/MCT for the financial support for the CESAM ((UID/AMB/50017/2019 and UIDB/50017/2020 + UIDP/50017/2020) and LAQV-REQUIMTE (UIDB/50006/2020) research units and to the FCT projects (PREVINE—FCT-PTDC/ASP-PES/29576/2017; P2020-PTDC/QUI-QOR/31770/2017 and P2020-PTDC/QEQ-SUP/5355/2014), through national funds and, where applicable, co-financed by the FEDER—Operational Thematic Program for Competitiveness and Internationalization−COMPETE 2020, within the PT2020 Partnership Agreement, and to the Portuguese NMR Network.
Publisher Copyright:
© 2022 by the authors.
PY - 2022/9/30
Y1 - 2022/9/30
N2 - The laboratorial available methods applied in plasma disinfection can induce damage in other blood components. Antimicrobial photodynamic therapy (aPDT) represents a promising approach and is approved for plasma and platelet disinfection using non-porphyrinic photosensitizers (PSs), such as methylene blue (MB). In this study, the photodynamic action of three cationic porphyrins (Tri-Py(+)-Me, Tetra-Py(+)-Me and Tetra-S-Py(+)-Me) towards viruses was evaluated under white light irradiation at an irradiance of 25 and 150 mW·cm−2, and the results were compared with the efficacy of the approved MB. None of the PSs caused hemolysis at the isotonic conditions, using a T4-like phage as a model of mammalian viruses. All porphyrins were more effective than MB in the photoinactivation of the T4-like phage in plasma. Moreover, the most efficient PS promoted a moderate inactivation rate of the T4-like phage in whole blood. Nevertheless, these porphyrins, such as MB, can be considered promising and safe PSs to photoinactivate viruses in blood plasma.
AB - The laboratorial available methods applied in plasma disinfection can induce damage in other blood components. Antimicrobial photodynamic therapy (aPDT) represents a promising approach and is approved for plasma and platelet disinfection using non-porphyrinic photosensitizers (PSs), such as methylene blue (MB). In this study, the photodynamic action of three cationic porphyrins (Tri-Py(+)-Me, Tetra-Py(+)-Me and Tetra-S-Py(+)-Me) towards viruses was evaluated under white light irradiation at an irradiance of 25 and 150 mW·cm−2, and the results were compared with the efficacy of the approved MB. None of the PSs caused hemolysis at the isotonic conditions, using a T4-like phage as a model of mammalian viruses. All porphyrins were more effective than MB in the photoinactivation of the T4-like phage in plasma. Moreover, the most efficient PS promoted a moderate inactivation rate of the T4-like phage in whole blood. Nevertheless, these porphyrins, such as MB, can be considered promising and safe PSs to photoinactivate viruses in blood plasma.
KW - Antimicrobial photodynamic therapy
KW - Blood
KW - Methylene blue
KW - Photosensitizers
KW - Plasma
KW - Porphyrins
KW - Viral inactivation
UR - http://www.scopus.com/inward/record.url?scp=85139916838&partnerID=8YFLogxK
U2 - 10.3390/ijms231911548
DO - 10.3390/ijms231911548
M3 - Article
C2 - 36232850
SN - 1661-6596
VL - 23
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 19
M1 - 11548
ER -