TY - JOUR
T1 - Antibiotic resistance in coagulase negative staphylococci isolated from wastewater and drinking water
AU - Faria, Cátia
AU - Vaz-Moreira, Ivone
AU - Serapicos, Eduarda
AU - Nunes, Olga C.
AU - Manaia, Célia M.
PY - 2009/6/1
Y1 - 2009/6/1
N2 - This study reports the antibiotic resistance patterns of coagulase negative staphylococci (CNS) isolated from a drinking water treatment plant (WTP), a drinking water distribution network, responsible for supplying water to the consumers (WDN), and a wastewater treatment plant (WWTP), responsible for receiving and treating domestic residual effluents. Genotyping and the 16S rRNA gene sequence analysis demonstrated a higher diversity of species both in the WTP (6 species/19 isolates) and WWTP (12 species/47 isolates) than in the WDN (6 species/172 isolates). Staphylococcus pasteuri and Staphylococcus epidermidis prevailed in the WTP and WDN and Staphylococcus saprophyticus in the WWTP. Staphylococci with reduced susceptibility (resistance or intermediary phenotype) to beta-lactams, tetracycline, clindamycin and erythromycin were observed in all types of water and belonged to the three major species groups. The highest resistance rate was found against erythromycin, presumably due to the presence of the efflux pump encoded by the determinant msrA, detected in the majority of the resistant isolates. This study demonstrates that antibiotic resistant CNS may colonize different types of water, namely drinking water fulfilling all the quality standards.
AB - This study reports the antibiotic resistance patterns of coagulase negative staphylococci (CNS) isolated from a drinking water treatment plant (WTP), a drinking water distribution network, responsible for supplying water to the consumers (WDN), and a wastewater treatment plant (WWTP), responsible for receiving and treating domestic residual effluents. Genotyping and the 16S rRNA gene sequence analysis demonstrated a higher diversity of species both in the WTP (6 species/19 isolates) and WWTP (12 species/47 isolates) than in the WDN (6 species/172 isolates). Staphylococcus pasteuri and Staphylococcus epidermidis prevailed in the WTP and WDN and Staphylococcus saprophyticus in the WWTP. Staphylococci with reduced susceptibility (resistance or intermediary phenotype) to beta-lactams, tetracycline, clindamycin and erythromycin were observed in all types of water and belonged to the three major species groups. The highest resistance rate was found against erythromycin, presumably due to the presence of the efflux pump encoded by the determinant msrA, detected in the majority of the resistant isolates. This study demonstrates that antibiotic resistant CNS may colonize different types of water, namely drinking water fulfilling all the quality standards.
KW - Antibiotic resistance
KW - Coagulase-negative staphylococci
KW - Drinking water
KW - Erythromycin
KW - msrA
UR - http://www.scopus.com/inward/record.url?scp=65049087164&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2009.02.034
DO - 10.1016/j.scitotenv.2009.02.034
M3 - Article
C2 - 19324394
AN - SCOPUS:65049087164
SN - 0048-9697
VL - 407
SP - 3876
EP - 3882
JO - Science of the Total Environment
JF - Science of the Total Environment
IS - 12
ER -
