TY - JOUR
T1 - Antibiotic resistance in European wastewater treatment plants mirrors the pattern of clinical antibiotic resistance prevalence
AU - Pärnänen, Katariina M. M.
AU - Narciso-da-Rocha, Carlos
AU - Kneis, David
AU - Berendonk, Thomas U.
AU - Cacace, Damiano
AU - Do, Thi Thuy
AU - Elpers, Christian
AU - Fatta-Kassinos, Despo
AU - Henriques, Isabel
AU - Jaeger, Thomas
AU - Karkman, Antti
AU - Martinez, Jose Luis
AU - Michael, Stella G.
AU - Michael-Kordatou, Irene
AU - O’Sullivan, Kristin
AU - Rodriguez-Mozaz, Sara
AU - Schwartz, Thomas
AU - Sheng, Hongjie
AU - Sørum, Henning
AU - Stedtfeld, Robert D.
AU - Tiedje, James M.
AU - Giustina, Saulo Varela Della
AU - Walsh, Fiona
AU - Vaz-Moreira, Ivone
AU - Virta, Marko
AU - Manaia, Célia M.
PY - 2019/3/27
Y1 - 2019/3/27
N2 - Integrated antibiotic resistance (AR) surveillance is one of the objectives of the World Health Organization global action plan on antimicrobial resistance. Urban wastewater treatment plants (UWTPs) are among the most important receptors and sources of environmental AR. On the basis of the consistent observation of an increasing north-to-south clinical AR prevalence in Europe, this study compared the influent and final effluent of 12 UWTPs located in seven countries (Portugal, Spain, Ireland, Cyprus, Germany, Finland, and Norway). Using highly parallel quantitative polymerase chain reaction, we analyzed 229 resistance genes and 25 mobile genetic elements. This first trans-Europe surveillance showed that UWTP AR profiles mirror the AR gradient observed in clinics. Antibiotic use, environmental temperature, and UWTP size were important factors related with resistance persistence and spread in the environment. These results highlight the need to implement regular surveillance and control measures, which may need to be appropriate for the geographic regions.
AB - Integrated antibiotic resistance (AR) surveillance is one of the objectives of the World Health Organization global action plan on antimicrobial resistance. Urban wastewater treatment plants (UWTPs) are among the most important receptors and sources of environmental AR. On the basis of the consistent observation of an increasing north-to-south clinical AR prevalence in Europe, this study compared the influent and final effluent of 12 UWTPs located in seven countries (Portugal, Spain, Ireland, Cyprus, Germany, Finland, and Norway). Using highly parallel quantitative polymerase chain reaction, we analyzed 229 resistance genes and 25 mobile genetic elements. This first trans-Europe surveillance showed that UWTP AR profiles mirror the AR gradient observed in clinics. Antibiotic use, environmental temperature, and UWTP size were important factors related with resistance persistence and spread in the environment. These results highlight the need to implement regular surveillance and control measures, which may need to be appropriate for the geographic regions.
UR - http://www.scopus.com/inward/record.url?scp=85064041764&partnerID=8YFLogxK
U2 - 10.1126/sciadv.aau9124
DO - 10.1126/sciadv.aau9124
M3 - Article
C2 - 30944853
AN - SCOPUS:85064041764
SN - 0019-5596
VL - 5
JO - Science Advances
JF - Science Advances
IS - 3
M1 - eaau9124
ER -
