TY - JOUR
T1 - Antimicrobial activity of pomegranate peel extracts performed by high pressure and enzymatic assisted extraction
AU - Alexandre, Elisabete M. C.
AU - Silva, Sara
AU - Santos, Sónia A. O.
AU - Silvestre, Armando J. D.
AU - Duarte, Maria F.
AU - Saraiva, Jorge A.
AU - Pintado, Manuela
N1 - Funding Information:
This work was supported by National Funds from Fundação para a Ciência e a Tecnologia - FCT through CBQF (FCT UID/Multi/ 50016/2013 ), CICECO ( FCT UID/CTM/50011/2013 ), QOPNA research Unit (FCT UID/ QUI/00062/2013 ), and to ICAAM research Unit (FCT UID/AGR/ 00115/2013 ), through national funds and where applicable co-financed by the FEDER, within the PT2020 Partnership Agreement. Author Elisabete Maria Cruz Alexandre also is grateful for the financial support of this work from “Fundação para a Ciência e Tecnologia - FCT” through the Post-doctoral Grant SFRH/BPD/ 95795/2013 . S.A.O. Santos thanks to the project AgroForWealth: Biorefining of agricultural and forest by-products and wastes: integrated strategic for valorization of resources towards society wealth and sustainability (CENTRO-01-0145-FEDER-000001), funded by Centro2020, through FEDER and PT2020, for the financial support.
Funding Information:
This work was supported by National Funds from Fundação para a Ciência e a Tecnologia - FCT through CBQF (FCT UID/Multi/50016/2013), CICECO (FCT UID/CTM/50011/2013), QOPNA research Unit (FCT UID/QUI/00062/2013), and to ICAAM research Unit (FCT UID/AGR/00115/2013), through national funds and where applicable co-financed by the FEDER, within the PT2020 Partnership Agreement. Author Elisabete Maria Cruz Alexandre also is grateful for the financial support of this work from “Fundação para a Ciência e Tecnologia - FCT” through the Post-doctoral Grant SFRH/BPD/95795/2013. S.A.O. Santos thanks to the project AgroForWealth: Biorefining of agricultural and forest by-products and wastes: integrated strategic for valorization of resources towards society wealth and sustainability (CENTRO-01-0145-FEDER-000001), funded by Centro2020, through FEDER and PT2020, for the financial support.
Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2019/1
Y1 - 2019/1
N2 - This study aimed to assess the effect of high pressure (300 and 600 MPa) and enzymatic extraction (pectinase and cellulase) on the phenolic compounds profile, antioxidant capacity and antimicrobial activity of extracts from pomegranate by-products. Antimicrobial activity against eight different strains of pathogenic and contaminant bacteria and against five beneficial bacteria including Lactobacillus and Bifidobacterium strains were determined. The maximum level of total phenolic content, as well as antioxidant capacity were observed at 300 MPa, however enzymatic extraction did not improve the extraction yields. Punicalagin isomers and bis-hexahydroxydiphenoyl-glucoside isomer were the most abundant phenolic compounds found in the extracts. All pomegranate peel extracts demonstrated selective antimicrobial activity against all pathogenic bacteria without affecting beneficial ones. Pressurized extracts presented lower minimum inhibitory concentration against Bacillus cereus and Pseudomonas aeruginosa and lower minimum bactericidal concentration against B. cereus, while, enzymatic extracts presented lower minimum bactericidal concentration for Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus and Listeria monocytogenes. Principal component analyses reveled that antioxidant activity and phenolic compounds content were strongly related with antimicrobial activity. Pomegranate peels extracts obtained by high pressure extraction could so be used as a source of high added-value bioactive compounds for antioxidant and antimicrobial applications.
AB - This study aimed to assess the effect of high pressure (300 and 600 MPa) and enzymatic extraction (pectinase and cellulase) on the phenolic compounds profile, antioxidant capacity and antimicrobial activity of extracts from pomegranate by-products. Antimicrobial activity against eight different strains of pathogenic and contaminant bacteria and against five beneficial bacteria including Lactobacillus and Bifidobacterium strains were determined. The maximum level of total phenolic content, as well as antioxidant capacity were observed at 300 MPa, however enzymatic extraction did not improve the extraction yields. Punicalagin isomers and bis-hexahydroxydiphenoyl-glucoside isomer were the most abundant phenolic compounds found in the extracts. All pomegranate peel extracts demonstrated selective antimicrobial activity against all pathogenic bacteria without affecting beneficial ones. Pressurized extracts presented lower minimum inhibitory concentration against Bacillus cereus and Pseudomonas aeruginosa and lower minimum bactericidal concentration against B. cereus, while, enzymatic extracts presented lower minimum bactericidal concentration for Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus and Listeria monocytogenes. Principal component analyses reveled that antioxidant activity and phenolic compounds content were strongly related with antimicrobial activity. Pomegranate peels extracts obtained by high pressure extraction could so be used as a source of high added-value bioactive compounds for antioxidant and antimicrobial applications.
KW - Antimicrobial activity
KW - Antioxidant activity
KW - Bioactive compounds
KW - Enzymatic assisted extraction
KW - High pressure extraction
KW - Minimum inhibitory and minimum bactericidal concentrations
KW - Phenolic compounds profile
KW - Pomegranate peel
UR - http://www.scopus.com/inward/record.url?scp=85052869921&partnerID=8YFLogxK
U2 - 10.1016/j.foodres.2018.08.044
DO - 10.1016/j.foodres.2018.08.044
M3 - Article
C2 - 30599929
AN - SCOPUS:85052869921
SN - 0963-9969
VL - 115
SP - 167
EP - 176
JO - Food research international
JF - Food research international
ER -