Antioxidant activity, phenolic profile, cytotoxicity and genotoxicity of plant extracts.

Adma N. F. Melo; Tiago B. Afonso; Tânia Ribeiro; Manuela Machado; Marta Carvalho; Márcio  Carocho; Freni Tavaria; Paula Teixeira; Lillian Barros; Manuela Pintado

Abstract

The outbreak of COVID-19 disease caused by SARS-CoV-2 forced the scientific world to search for new alternatives to help control the virus. Plant extracts have natural compounds that might provide a starting point for the research on the use of plants as an excellent source of new antiviral agents against viruses, including COVID-19 to be included in disinfectants, fabrics or other materials. In this study, the polyphenols content (Folin-Ciocalteu), antioxidant capacity (DPPH, ABTS and ORAC) and the phenolic profile (HPLC) of different hydroethanolic (ethanol:H2O 50:50 v/v) extracts of medicinal plants cultivated under controlled conditions in Portugal (echinacea, rosemary, laurel, thyme and rock rose) were determined, as well as the cytotoxicity effect against a keratinocyte cell line using cell viability assay by PrestoBlue and genotoxicity effect using the AMES test. According to the results, total phenolic content ranged from 204.54 ± 1.78 / 274.20 ± 3.14 (mg EAG/g extract) with the rock rose extract presenting the highest content (p < 0.05). The extracts showed a good antioxidant capacity demonstrated by the high values found for ORAC, which ranged 2855.03 ± 9.75 / 5285.35 ± 60.04 µMol Trolox/mg extract. HPLC analysis revealed the presence of different compounds in the extracts such as the kaempferol-O-glucuronide, catechin, protocatechuic acid and galloyl glucoside, representing a potential source of bioactive components with antioxidant capacity. No toxicity was observed towards the keratinocyte cells and none of them showed mutagenic effects. Based on the results of safety and high polyphenols content of the extracts they demonstrate a great potential as antimicrobial agents. This will allow the design of new experiments aimed at evaluating the antiviral activity of these extracts, especially against SARS-CoV-2.

Original language	English
Pages	302-302
Number of pages	1
Publication status	Published - 2021
Event	Congress of Microbiology and Biotechnology 2021: MICROBIOTEC'21 - Universidade Nova de Lisboa, Lisboa, Portugal Duration: 23 Nov 2021 → 26 Nov 2021 https://microbiotec21.organideia.pt

Conference

Conference	Congress of Microbiology and Biotechnology 2021
Abbreviated title	Microbiotec'21
Country/Territory	Portugal
City	Lisboa
Period	23/11/21 → 26/11/21
Internet address	https://microbiotec21.organideia.pt

Access to Document

60217873Final published version, 208 KBLicence: Unspecified

1 Active

CBQF Multianual Funding: Centre for Biotechnology and Fine Chemistry
Cortez, J., Pintado, M. M., Morais, A. M. M. B., Ribeiro, A. B., Ferreira, A. C., Castro, P., Villamor, A. L., Oliveira, A. L., Oliveira, A. L. S., Amaro, A. L., Paulo, A., Gomes, A. M., Marques, A., Carvalho, A. P., Monforte, A. R., Madureira, A. R., Oliveira, A. S., Ferreira, A. S., Rangel, A. O. S. S., Oliveira, C., Cálix, C., Pereira, C. F., Santos, C. S. D., Amorim, C. L., Oliveira, C., Manaia, C., Silva, C. L. M., Monteiro, C. M., Machado, D., Campos, D., Oliveira, D., Valente, D., Cardoso, E., Costa, E. M. A. D., Pinto, E., Alexandre, E., Carsanba, E., Coscueta, E., Raposo, M. F. D. J., Tavaria, F., Voss, G. B., Moreira, H., Campos, I., Tomada, I., Moreira, I. S., Vaz-Moreira, I., Fundo, J. F., Barbosa, J., Pereira, J. O., Durão, J. R. D. O., Costa, J. R., Fernandes, J. C., Ferreira, J., Silva, J. A., Couto, J. A., Oliveira, L., Pimentel, L., Rodríguez-Alcalá, L. M., Hogg, C., Poças, M. D. F., Miller, F. A., Brassesco, M. E., Mota, I., Ramos, I. N., Vasconcelos, I., Monteiro, M. J., Amorim, M., Roriz, M., Silva, M., Correia, M., Silva, M. N. D., Alves, M., Vasconcelos, M., Ramos, O. L., Costa, P., Gibbs, P., Teixeira, P., Ramos, P., Rodrigues, P. M., Carvalho, P., Mesquita, R., Barros, R., Morais, R. M. S. C., Magalhães, R. M. B. D. S., Borges, S. C. F., Correia, S., Silva, S. B. D., Silva, S., Moreira, S. A. M., Pedrosa, S., Pereira, S., Vidigal, S., Pedro, T., Ribeiro, T., Deuchande, T., Brandão, T. R. S., Resende, T., Hogg, T., Ferreira, V., Viseras, A. D., Soares, A. M. S., Oliveira, A. S., Uribe, B., Pais-Vieira, C., Ferreira, C., Sousa, C. C. S., Kumla, D., Carsanba, E., Lopes, G. L. L., Ribeiro, I. M. M. V. P., Cruz, I. B. D., Barbosa, J. C., Oliveira, L., Mendes, M. A. R., Boas, A. V., Cassoni, A. C., Lopes, A., Pintado, A. I. E., Sousa, A. P., Faustino, A., Salsinha, A. S., Couto, A. T., Macieira, A., Horta, B. M., Silva, C. M. E., Rodrigues, C. M., Bernardes, B. G., Silva, B. I. Q. L. D., Costa, C., Costa, C. M., Serafim, C., Santos, D., Antunes, F., Vieira, E., Ferreira, H., Moreira, I. A., Vieira, I. R. D. F., Rocha, J., Henggeler , J., Miranda, J., Oliveira, M., Lopes, M. T. B. V., Silva, N., Carvalho, N., Costa, P., Nova, P., Sousa, P., Silva, P., Gómez-García, R., Freixo, R., Marçal, S., Cunha, S. A., Sousa, S., Pereira, S. A., Ghalamara, S., Vilela, T., Lopes, A., Lopes, A. I. R., Linhares, A. L. S., Boas, A. M. M. V., Teixeira, A. M. R., Mendes, A. R. M., Oliveira, A. S., Sousa, A. S. D. S., Sousa, A. S. D. S., Martins, A. S. P. D. P., Veiga, A., Bernardes, B. G., Silva, B. I. Q. L. D., Fernandes, A. S., Miranda, C., Dias, C. L. A., Neto, C. C., Castro, C. M. O., Oliveira, D. F., Rodrigues, D., Magalhães, D. D. S., Almeida, D., Castro, D., Costa, E. C. L. D., Darú, F. A., Bastos, F., Teixeira, F. D. S., Ferreira, F., Fortunato, G., Araújo-Rodrigues, H., Osorio Pérez, J. R., Silveira, J. F., Silva, J. A., Soares, J. C., Carvalho, L. C., Castro, L. M. G., Machado, M., Carvalho, M. J., Nazareth, M., Veiga, M., Coelho, M., Carvalho, M. I. P. D., Mendes, M., Ramos, M. A. M., Silva, T., Resende, T., Fontes, A. L., Neves, D. I. S. C. D., Cachetas, D. M., Silva, G. A. D. R. & Morais, R. M. S. C.
1/01/20 → 31/12/23
Project: Research

1 Poster

Antioxidant activity, phenolic profile, cytotoxicity and genotoxicity of plant extracts.
Melo, A. N. F. D., Afonso, T. B., Ribeiro, T., Machado, M., Carvalho, M. I. P. D., Carocho, M., Tavaria, F., Carvalho, M., Teixeira, P., Barros, L. & Pintado, M., 2021, p. 1-1. 1 p.
Research output: Contribution to conference › Poster › peer-review

Open Access
File

Cite this

@conference{2613b54a42514caebf2f0324f9f4a134,

title = "Antioxidant activity, phenolic profile, cytotoxicity and genotoxicity of plant extracts.",

abstract = " The outbreak of COVID-19 disease caused by SARS-CoV-2 forced the scientific world to search for new alternatives to help control the virus. Plant extracts have natural compounds that might provide a starting point for the research on the use of plants as an excellent source of new antiviral agents against viruses, including COVID-19 to be included in disinfectants, fabrics or other materials. In this study, the polyphenols content (Folin-Ciocalteu), antioxidant capacity (DPPH, ABTS and ORAC) and the phenolic profile (HPLC) of different hydroethanolic (ethanol:H2O 50:50 v/v) extracts of medicinal plants cultivated under controlled conditions in Portugal (echinacea, rosemary, laurel, thyme and rock rose) were determined, as well as the cytotoxicity effect against a keratinocyte cell line using cell viability assay by PrestoBlue and genotoxicity effect using the AMES test. According to the results, total phenolic content ranged from 204.54 ± 1.78 / 274.20 ± 3.14 (mg EAG/g extract) with the rock rose extract presenting the highest content (p < 0.05). The extracts showed a good antioxidant capacity demonstrated by the high values found for ORAC, which ranged 2855.03 ± 9.75 / 5285.35 ± 60.04 µMol Trolox/mg extract. HPLC analysis revealed the presence of different compounds in the extracts such as the kaempferol-O-glucuronide, catechin, protocatechuic acid and galloyl glucoside, representing a potential source of bioactive components with antioxidant capacity. No toxicity was observed towards the keratinocyte cells and none of them showed mutagenic effects. Based on the results of safety and high polyphenols content of the extracts they demonstrate a great potential as antimicrobial agents. This will allow the design of new experiments aimed at evaluating the antiviral activity of these extracts, especially against SARS-CoV-2. ",

author = "Melo, {Adma N. F.} and Afonso, {Tiago B.} and T{\^a}nia Ribeiro and Manuela Machado and Marta Carvalho and M{\'a}rcio Carocho and Freni Tavaria and Paula Teixeira and Lillian Barros and Manuela Pintado",

year = "2021",

language = "English",

pages = "302--302",

note = "Congress of Microbiology and Biotechnology 2021, Microbiotec'21 ; Conference date: 23-11-2021 Through 26-11-2021",

url = "https://microbiotec21.organideia.pt",

}

TY - CONF

T1 - Antioxidant activity, phenolic profile, cytotoxicity and genotoxicity of plant extracts.

AU - Melo, Adma N. F.

AU - Afonso, Tiago B.

AU - Ribeiro, Tânia

AU - Machado, Manuela

AU - Carvalho, Marta

AU - Carocho, Márcio

AU - Tavaria, Freni

AU - Teixeira, Paula

AU - Barros, Lillian

AU - Pintado, Manuela

PY - 2021

Y1 - 2021

N2 - The outbreak of COVID-19 disease caused by SARS-CoV-2 forced the scientific world to search for new alternatives to help control the virus. Plant extracts have natural compounds that might provide a starting point for the research on the use of plants as an excellent source of new antiviral agents against viruses, including COVID-19 to be included in disinfectants, fabrics or other materials. In this study, the polyphenols content (Folin-Ciocalteu), antioxidant capacity (DPPH, ABTS and ORAC) and the phenolic profile (HPLC) of different hydroethanolic (ethanol:H2O 50:50 v/v) extracts of medicinal plants cultivated under controlled conditions in Portugal (echinacea, rosemary, laurel, thyme and rock rose) were determined, as well as the cytotoxicity effect against a keratinocyte cell line using cell viability assay by PrestoBlue and genotoxicity effect using the AMES test. According to the results, total phenolic content ranged from 204.54 ± 1.78 / 274.20 ± 3.14 (mg EAG/g extract) with the rock rose extract presenting the highest content (p < 0.05). The extracts showed a good antioxidant capacity demonstrated by the high values found for ORAC, which ranged 2855.03 ± 9.75 / 5285.35 ± 60.04 µMol Trolox/mg extract. HPLC analysis revealed the presence of different compounds in the extracts such as the kaempferol-O-glucuronide, catechin, protocatechuic acid and galloyl glucoside, representing a potential source of bioactive components with antioxidant capacity. No toxicity was observed towards the keratinocyte cells and none of them showed mutagenic effects. Based on the results of safety and high polyphenols content of the extracts they demonstrate a great potential as antimicrobial agents. This will allow the design of new experiments aimed at evaluating the antiviral activity of these extracts, especially against SARS-CoV-2.

AB - The outbreak of COVID-19 disease caused by SARS-CoV-2 forced the scientific world to search for new alternatives to help control the virus. Plant extracts have natural compounds that might provide a starting point for the research on the use of plants as an excellent source of new antiviral agents against viruses, including COVID-19 to be included in disinfectants, fabrics or other materials. In this study, the polyphenols content (Folin-Ciocalteu), antioxidant capacity (DPPH, ABTS and ORAC) and the phenolic profile (HPLC) of different hydroethanolic (ethanol:H2O 50:50 v/v) extracts of medicinal plants cultivated under controlled conditions in Portugal (echinacea, rosemary, laurel, thyme and rock rose) were determined, as well as the cytotoxicity effect against a keratinocyte cell line using cell viability assay by PrestoBlue and genotoxicity effect using the AMES test. According to the results, total phenolic content ranged from 204.54 ± 1.78 / 274.20 ± 3.14 (mg EAG/g extract) with the rock rose extract presenting the highest content (p < 0.05). The extracts showed a good antioxidant capacity demonstrated by the high values found for ORAC, which ranged 2855.03 ± 9.75 / 5285.35 ± 60.04 µMol Trolox/mg extract. HPLC analysis revealed the presence of different compounds in the extracts such as the kaempferol-O-glucuronide, catechin, protocatechuic acid and galloyl glucoside, representing a potential source of bioactive components with antioxidant capacity. No toxicity was observed towards the keratinocyte cells and none of them showed mutagenic effects. Based on the results of safety and high polyphenols content of the extracts they demonstrate a great potential as antimicrobial agents. This will allow the design of new experiments aimed at evaluating the antiviral activity of these extracts, especially against SARS-CoV-2.

M3 - Abstract

SP - 302

EP - 302

T2 - Congress of Microbiology and Biotechnology 2021

Y2 - 23 November 2021 through 26 November 2021

ER -

Antioxidant activity, phenolic profile, cytotoxicity and genotoxicity of plant extracts.

Abstract

Conference

Access to Document

Fingerprint

Projects

CBQF Multianual Funding: Centre for Biotechnology and Fine Chemistry

Research output

Antioxidant activity, phenolic profile, cytotoxicity and genotoxicity of plant extracts.

Cite this