TY - JOUR
T1 - Antioxidant potential of two red seaweeds from the Brazilian coasts
AU - Souza, Bartolomeu W. S.
AU - Cerqueira, Miguel A.
AU - Martins, Joana T.
AU - Quintas, Mafalda A. C.
AU - Ferreira, António C. S.
AU - Teixeira, José A.
AU - Vicente, António A.
PY - 2011/5/25
Y1 - 2011/5/25
N2 - In this work, in vitro antioxidant activity of two Brazilian red seaweeds, Gracilaria birdiae and Gracilaria cornea, was characterized. The total phenolic content, the radical-scavenging activity and the antioxidant activity were determined in two solvent extracts of the algae. Liquid chromatography-mass spectrometry (LC-MS/MS) allowed identification of important antioxidant compounds. The ethanol extract of G birdiae was found to have the highest value of total phenolic content: 1.13 mg of gallic acid equiv (GAE)/g of extract. The radical-scavenging activity of G. birdiae and G cornea extracts has been evaluated at different extract concentrations; the IC50 values of ethanolic extracts of G cornea and G birdiae were 0.77 and 0.76 mg mL, respectively while for methanolic extracts, the IC50 values of G cornea and G birdiae were 0.86 and 0.76 mg mL-1, respectively. The antioxidant activities of these two seaweeds' extracts as assessed by the β-carotene-linoleic acid assay were equally high, achieving values of β-carotene oxidation inhibition of up to 40%. Finally, in the methanolic extracts, LC-MS/MS allowed identification in both algae of two important antioxidants: apigenin and gallic acid.
AB - In this work, in vitro antioxidant activity of two Brazilian red seaweeds, Gracilaria birdiae and Gracilaria cornea, was characterized. The total phenolic content, the radical-scavenging activity and the antioxidant activity were determined in two solvent extracts of the algae. Liquid chromatography-mass spectrometry (LC-MS/MS) allowed identification of important antioxidant compounds. The ethanol extract of G birdiae was found to have the highest value of total phenolic content: 1.13 mg of gallic acid equiv (GAE)/g of extract. The radical-scavenging activity of G. birdiae and G cornea extracts has been evaluated at different extract concentrations; the IC50 values of ethanolic extracts of G cornea and G birdiae were 0.77 and 0.76 mg mL, respectively while for methanolic extracts, the IC50 values of G cornea and G birdiae were 0.86 and 0.76 mg mL-1, respectively. The antioxidant activities of these two seaweeds' extracts as assessed by the β-carotene-linoleic acid assay were equally high, achieving values of β-carotene oxidation inhibition of up to 40%. Finally, in the methanolic extracts, LC-MS/MS allowed identification in both algae of two important antioxidants: apigenin and gallic acid.
KW - Antioxidant
KW - Phenolic content
KW - Seaweeds
UR - http://www.scopus.com/inward/record.url?scp=79957960929&partnerID=8YFLogxK
U2 - 10.1021/jf200999n
DO - 10.1021/jf200999n
M3 - Article
C2 - 21491929
AN - SCOPUS:79957960929
SN - 0021-8561
VL - 59
SP - 5589
EP - 5594
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 10
ER -