Antiulcerogenic activity of peptide concentrates obtained from hydrolysis of whey proteins by proteases from Cynara cardunculus

T. G. Tavares; K. M. Monteiro; A. Possenti; M. E. Pintado; J. E. Carvalho; F. X. Malcata

doi:10.1016/j.idairyj.2011.06.004

Antiulcerogenic activity of peptide concentrates obtained from hydrolysis of whey proteins by proteases from Cynara cardunculus

T. G. Tavares, K. M. Monteiro, A. Possenti, M. E. Pintado, J. E. Carvalho, F. X. Malcata^*

^*Corresponding author for this work

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Abstract

Peptide concentrates generated by hydrolysis of whey with aqueous extracts of flowers of Cynara cardunculus were studied for possible protection of the stomach mucosa against ulcerative lesions caused by oral administration of absolute ethanol. Both the whole peptide fraction obtained via hydrolysis of whey protein concentrate (peptide concentrate, PepC) and its fraction below 3kDa (PepCF) were able to reduce gastric injuries to significant levels (p<0.05). Single-dose experiments, using 100mgkg-1 body weight (bw) of either PepCF or PepC, led to 68.5% and 37.4% protection, respectively - which compare well with 93.4% protection by 200mgkg-1bw carbenoxolone (a positive control). No dose-response correlation could be demonstrated. Gastric cytoprotection by PepCF appears to depend on sulphydryl-containing moieties, whereas PepC likely protects the gastric mucosa via the prostaglandin cycle and production of nitric oxide.

Original language	English
Pages (from-to)	934-939
Number of pages	6
Journal	International Dairy Journal
Volume	21
Issue number	12
DOIs	https://doi.org/10.1016/j.idairyj.2011.06.004
Publication status	Published - Dec 2011

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10.1016/j.idairyj.2011.06.004

http://hdl.handle.net/10400.14/7965

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title = "Antiulcerogenic activity of peptide concentrates obtained from hydrolysis of whey proteins by proteases from Cynara cardunculus",

abstract = "Peptide concentrates generated by hydrolysis of whey with aqueous extracts of flowers of Cynara cardunculus were studied for possible protection of the stomach mucosa against ulcerative lesions caused by oral administration of absolute ethanol. Both the whole peptide fraction obtained via hydrolysis of whey protein concentrate (peptide concentrate, PepC) and its fraction below 3kDa (PepCF) were able to reduce gastric injuries to significant levels (p<0.05). Single-dose experiments, using 100mgkg-1 body weight (bw) of either PepCF or PepC, led to 68.5% and 37.4% protection, respectively - which compare well with 93.4% protection by 200mgkg-1bw carbenoxolone (a positive control). No dose-response correlation could be demonstrated. Gastric cytoprotection by PepCF appears to depend on sulphydryl-containing moieties, whereas PepC likely protects the gastric mucosa via the prostaglandin cycle and production of nitric oxide.",

author = "Tavares, {T. G.} and Monteiro, {K. M.} and A. Possenti and Pintado, {M. E.} and Carvalho, {J. E.} and Malcata, {F. X.}",

year = "2011",

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T1 - Antiulcerogenic activity of peptide concentrates obtained from hydrolysis of whey proteins by proteases from Cynara cardunculus

AU - Tavares, T. G.

AU - Monteiro, K. M.

AU - Possenti, A.

AU - Pintado, M. E.

AU - Carvalho, J. E.

AU - Malcata, F. X.

PY - 2011/12

Y1 - 2011/12

N2 - Peptide concentrates generated by hydrolysis of whey with aqueous extracts of flowers of Cynara cardunculus were studied for possible protection of the stomach mucosa against ulcerative lesions caused by oral administration of absolute ethanol. Both the whole peptide fraction obtained via hydrolysis of whey protein concentrate (peptide concentrate, PepC) and its fraction below 3kDa (PepCF) were able to reduce gastric injuries to significant levels (p<0.05). Single-dose experiments, using 100mgkg-1 body weight (bw) of either PepCF or PepC, led to 68.5% and 37.4% protection, respectively - which compare well with 93.4% protection by 200mgkg-1bw carbenoxolone (a positive control). No dose-response correlation could be demonstrated. Gastric cytoprotection by PepCF appears to depend on sulphydryl-containing moieties, whereas PepC likely protects the gastric mucosa via the prostaglandin cycle and production of nitric oxide.

AB - Peptide concentrates generated by hydrolysis of whey with aqueous extracts of flowers of Cynara cardunculus were studied for possible protection of the stomach mucosa against ulcerative lesions caused by oral administration of absolute ethanol. Both the whole peptide fraction obtained via hydrolysis of whey protein concentrate (peptide concentrate, PepC) and its fraction below 3kDa (PepCF) were able to reduce gastric injuries to significant levels (p<0.05). Single-dose experiments, using 100mgkg-1 body weight (bw) of either PepCF or PepC, led to 68.5% and 37.4% protection, respectively - which compare well with 93.4% protection by 200mgkg-1bw carbenoxolone (a positive control). No dose-response correlation could be demonstrated. Gastric cytoprotection by PepCF appears to depend on sulphydryl-containing moieties, whereas PepC likely protects the gastric mucosa via the prostaglandin cycle and production of nitric oxide.

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JO - International Dairy Journal

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ER -

Antiulcerogenic activity of peptide concentrates obtained from hydrolysis of whey proteins by proteases from Cynara cardunculus

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