Application of the resazurin cell viability assay to monitor escherichia coli and salmonella typhimurium inactivation mediated by phages

Pedro Costa, Ana T. P. C. Gomes, Márcia Braz, Carla Pereira, Adelaide Almeida*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Bacterial inactivation using bacteriophages (or phages) has emerged as an effective solution for bacterial infections, but the screening methods used to evaluate the effectiveness of the phages to inactivate bacteria are not fast, reliable or precise enough. The efficiency of bacterial inac-tivation by phages has been evaluated by monitoring bacterial concentration either by counting col-ony-forming units (CFU), a laborious and time-consuming method, or by monitoring the optical density (OD), a less sensitive method. In this study, the resazurin cell viability assay was used to monitor the viability of bacteria from different genera during the inactivation by different phages, and the results were compared with the standard methods used to assess bacterial inactivation. The results showed that the resazurin colorimetric cell viability assay produces similar results to the standard method of colony-counting and giving, and also more sensitive results than the OD method. The resazurin assay can be used to quickly obtain the results of the cell viability effect profile using two different bacterial strains and several different phages at the same time, which is extremely valuable in screening studies. Moreover, this methodology is established as an effective, accurate and rapid method when compared to the ones widely used to monitor bacterial inactiva-tion mediated by phages.
Original languageEnglish
Article number974
JournalAntibiotics
Volume10
Issue number8
DOIs
Publication statusPublished - Aug 2021
Externally publishedYes

Keywords

  • Antimicrobial therapy
  • Bacteriophages
  • Cell viability
  • Pathogenic bacteria
  • Resazurin

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