Assessment of cardiovascular disease risk using immunosensors for determination of C-reactive protein levels in serum and saliva: a pilot study

Celine I. Justino; Kátia Duarte; Susana Lucas; Paulo Chaves; Paulo Bettencourt; Ana Cristina Freitas; Ruth Pereira; Susana Cardoso; Armando C. Duarte; Teresa A. Rocha-Santos

doi:10.4155/bio.14.12

Assessment of cardiovascular disease risk using immunosensors for determination of C-reactive protein levels in serum and saliva: a pilot study

Celine I. Justino^*, Kátia Duarte, Susana Lucas, Paulo Chaves, Paulo Bettencourt, Ana Cristina Freitas, Ruth Pereira, Susana Cardoso, Armando C. Duarte, Teresa A. Rocha-Santos

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Background: Disposable immunosensors based on field effect transistors with single-walled carbon nanotubes (NTFET) were applied for the first time to clinical samples of undiluted blood serum and saliva for the determination of C-reactive protein (CRP), and validated by comparison with ELISA. Results: The NTFET showed comparable analytical performance with the ELISA when applied to clinical samples, which means that NTFET can be used as an alternative to ELISA. Also, a high correlation between the serum and salivary CRP levels was found with the NTFET, which means that saliva could be used based on a noninvasive sampling as an alternative fluid to blood serum. The establishment of a new range of CRP levels based on saliva was also found. Conclusion: The monitoring of CRP in saliva samples by disposable immunosensors could be a valuable approach for the improvement of healthcare services, considering the worldwide increased incidence of cardiovascular diseases.

Original language	English
Pages (from-to)	1459-1470
Number of pages	12
Journal	Bioanalysis
Volume	6
Issue number	11
DOIs	https://doi.org/10.4155/bio.14.12
Publication status	Published - Jun 2014
Externally published	Yes

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title = "Assessment of cardiovascular disease risk using immunosensors for determination of C-reactive protein levels in serum and saliva: a pilot study",

abstract = "Background: Disposable immunosensors based on field effect transistors with single-walled carbon nanotubes (NTFET) were applied for the first time to clinical samples of undiluted blood serum and saliva for the determination of C-reactive protein (CRP), and validated by comparison with ELISA. Results: The NTFET showed comparable analytical performance with the ELISA when applied to clinical samples, which means that NTFET can be used as an alternative to ELISA. Also, a high correlation between the serum and salivary CRP levels was found with the NTFET, which means that saliva could be used based on a noninvasive sampling as an alternative fluid to blood serum. The establishment of a new range of CRP levels based on saliva was also found. Conclusion: The monitoring of CRP in saliva samples by disposable immunosensors could be a valuable approach for the improvement of healthcare services, considering the worldwide increased incidence of cardiovascular diseases.",

author = "Justino, {Celine I.} and K{\'a}tia Duarte and Susana Lucas and Paulo Chaves and Paulo Bettencourt and Freitas, {Ana Cristina} and Ruth Pereira and Susana Cardoso and Duarte, {Armando C.} and Rocha-Santos, {Teresa A.}",

year = "2014",

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language = "English",

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T1 - Assessment of cardiovascular disease risk using immunosensors for determination of C-reactive protein levels in serum and saliva

T2 - a pilot study

AU - Justino, Celine I.

AU - Duarte, Kátia

AU - Lucas, Susana

AU - Chaves, Paulo

AU - Bettencourt, Paulo

AU - Freitas, Ana Cristina

AU - Pereira, Ruth

AU - Cardoso, Susana

AU - Duarte, Armando C.

AU - Rocha-Santos, Teresa A.

PY - 2014/6

Y1 - 2014/6

N2 - Background: Disposable immunosensors based on field effect transistors with single-walled carbon nanotubes (NTFET) were applied for the first time to clinical samples of undiluted blood serum and saliva for the determination of C-reactive protein (CRP), and validated by comparison with ELISA. Results: The NTFET showed comparable analytical performance with the ELISA when applied to clinical samples, which means that NTFET can be used as an alternative to ELISA. Also, a high correlation between the serum and salivary CRP levels was found with the NTFET, which means that saliva could be used based on a noninvasive sampling as an alternative fluid to blood serum. The establishment of a new range of CRP levels based on saliva was also found. Conclusion: The monitoring of CRP in saliva samples by disposable immunosensors could be a valuable approach for the improvement of healthcare services, considering the worldwide increased incidence of cardiovascular diseases.

AB - Background: Disposable immunosensors based on field effect transistors with single-walled carbon nanotubes (NTFET) were applied for the first time to clinical samples of undiluted blood serum and saliva for the determination of C-reactive protein (CRP), and validated by comparison with ELISA. Results: The NTFET showed comparable analytical performance with the ELISA when applied to clinical samples, which means that NTFET can be used as an alternative to ELISA. Also, a high correlation between the serum and salivary CRP levels was found with the NTFET, which means that saliva could be used based on a noninvasive sampling as an alternative fluid to blood serum. The establishment of a new range of CRP levels based on saliva was also found. Conclusion: The monitoring of CRP in saliva samples by disposable immunosensors could be a valuable approach for the improvement of healthcare services, considering the worldwide increased incidence of cardiovascular diseases.

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ER -

Assessment of cardiovascular disease risk using immunosensors for determination of C-reactive protein levels in serum and saliva: a pilot study

Abstract

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