Assessment of cardiovascular disease risk using immunosensors for determination of C-reactive protein levels in serum and saliva: a pilot study

Celine I. Justino*, Kátia Duarte, Susana Lucas, Paulo Chaves, Paulo Bettencourt, Ana Cristina Freitas, Ruth Pereira, Susana Cardoso, Armando C. Duarte, Teresa A. Rocha-Santos

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Background: Disposable immunosensors based on field effect transistors with single-walled carbon nanotubes (NTFET) were applied for the first time to clinical samples of undiluted blood serum and saliva for the determination of C-reactive protein (CRP), and validated by comparison with ELISA. Results: The NTFET showed comparable analytical performance with the ELISA when applied to clinical samples, which means that NTFET can be used as an alternative to ELISA. Also, a high correlation between the serum and salivary CRP levels was found with the NTFET, which means that saliva could be used based on a noninvasive sampling as an alternative fluid to blood serum. The establishment of a new range of CRP levels based on saliva was also found. Conclusion: The monitoring of CRP in saliva samples by disposable immunosensors could be a valuable approach for the improvement of healthcare services, considering the worldwide increased incidence of cardiovascular diseases.
Original languageEnglish
Pages (from-to)1459-1470
Number of pages12
JournalBioanalysis
Volume6
Issue number11
DOIs
Publication statusPublished - Jun 2014
Externally publishedYes

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