Assessment of copper and zinc salts as selectors of antibiotic resistance in gram-negative bacteria

Cristina Becerra-Castro, Rita A. Machado, Ivone Vaz-Moreira, Célia M. Manaia*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

Some metals are nowadays considered environmental pollutants. Although some, like Cu and Zn, are essential for microorganisms, at high concentrations they can be toxic or exert selective pressures on bacteria. This study aimed to assess the potential of Cu or Zn as selectors of specific bacterial populations thriving in wastewater. Populations of Escherichia coli recovered on metal-free and metal-supplemented culture medium were compared based on antibiotic resistance phenotype and other traits. In addition, the bacterial groups enriched after successive transfers in metal-supplemented culture medium were identified. At a concentration of 1. mM, Zn produced a stronger inhibitory effect than Cu on the culturability of Enterobacteriaceae. It was suggested that Zn selected populations with increased resistance prevalence to sulfamethoxazole or ciprofloxacin. In non-selective culture media, Zn or Cu selected for mono-species populations of ubiquitous Betaproteobacteria and Flavobacteriia, such as Ralstonia pickettii or Elizabethkingia anophelis, yielding multidrug resistance profiles including resistance against carbapenems and third generation cephalosporins, confirming the potential of Cu or Zn as selectors of antibiotic resistant bacteria.
Original languageEnglish
Pages (from-to)367-372
Number of pages6
JournalScience of the Total Environment
Volume530-531
DOIs
Publication statusPublished - 15 Oct 2015

Keywords

  • Antibiotic resistance selectors
  • E. coli
  • Metals
  • Selective pressure
  • Wastewater

Fingerprint

Dive into the research topics of 'Assessment of copper and zinc salts as selectors of antibiotic resistance in gram-negative bacteria'. Together they form a unique fingerprint.

Cite this