Bacillus purgationiresistans sp. nov., isolated from a drinking-water treatment plant

A Gram-positive, aerobic, non-motile, endospore-forming rod, designated DS22T, was isolated from a drinking-water treatment plant. Cells were catalase- and oxidase-positive. Growth occurred at 15-37 oC, at pH 7-10 and with,8% (w/v) NaCl (optimum growth: 30 6C, pH 7-8 and 1-3% NaCl). The major respiratory quinone was menaquinone 7, the G+C content of the genomic DNA was 36.5 mol% and the cell wall contained meso-diaminopimelic acid. On the basis of 16S rRNA gene sequence analysis, strain DS22T was a member of the genus Bacillus. Its closest phylogenetic neighbours were Bacillus horneckiae NRRL B-59162T (98.5% 16S rRNA gene sequence similarity), Bacillus oceanisediminis H2 T (97.9 %), Bacillus infantis SMC 4352-1 T (97.4 %), Bacillus firmus IAM 12464 T (96.8 %) and Bacillus muralis LMG 20238 T (96.8 %). DNA-DNA hybridization, and biochemical and physiological characterization allowed the differentiation of strain DS22 T from its closest phylogenetic neighbours. The data supports the proposal of a novel species, Bacillus purgationiresistans sp. nov.; the type strain is DS22 T (5DSM 23494T5NRRL B-59432T5LMG 25783 T).