TY - JOUR
T1 - Bacterial lineages putatively associated with the dissemination of antibiotic resistance genes in a full-scale urban wastewater treatment plant
AU - Narciso-da-Rocha, Carlos
AU - Rocha, Jaqueline
AU - Vaz-Moreira, Ivone
AU - Lira, Felipe
AU - Tamames, Javier
AU - Henriques, Isabel
AU - Martinez, José Luis
AU - Manaia, Célia M.
N1 - Funding Information:
This work was supported by National Funds from FCT – Fundação para a Ciência e a Tecnologia through the WaterJPI/0001/2013 project STARE – “Stopping Antibiotic Resistance Evolution”. Furthermore, thanks are due for the financial support to CESAM, to FCT/MCTES through national funds (PIDDAC), and the co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020 (UID/AMB/50017 - POCI-01-0145-FEDER-007638). Authors would also like to thank the scientific collaboration under the FCT project UID/Multi/50016/2013. IVM was supported by the FCT grant (SFRH/BPD/87360/2012), CNR by the FCT grant SFRH/BD/97131/2013, IH by FCT contract IF/00492/2013, JR by the International PhD Programme in Biotechnology – BIOTECH.DOC, NORTE-08-5369-FSE-000007, JT by project CTM2013-48292-C3-2-R (MINECO/FEDER), Spain.
Funding Information:
This work was supported by National Funds from FCT – Fundação para a Ciência e a Tecnologia through the WaterJPI/0001/2013 project STARE – “Stopping Antibiotic Resistance Evolution”. Furthermore, thanks are due for the financial support to CESAM , to FCT/MCTES through national funds (PIDDAC), and the co-funding by the FEDER , within the PT2020 Partnership Agreement and Compete 2020 ( UID/AMB/50017 - POCI-01-0145-FEDER-007638 ). Authors would also like to thank the scientific collaboration under the FCT project UID/Multi/50016/2013. IVM was supported by the FCT grant ( SFRH/BPD/87360/2012 ), CNR by the FCT grant SFRH/BD/97131/2013 , IH by FCT contract IF/00492/2013 , JR by the International PhD Programme in Biotechnology – BIOTECH.DOC , NORTE-08-5369-FSE-000007 , JT by project CTM2013-48292-C3-2-R ( MINECO/FEDER ), Spain.
Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/9
Y1 - 2018/9
N2 - Urban wastewater treatment plants (UWTPs) are reservoirs of antibiotic resistance. Wastewater treatment changes the bacterial community and inevitably impacts the fate of antibiotic resistant bacteria and antibiotic resistance genes (ARGs). Some bacterial groups are major carriers of ARGs and hence, their elimination during wastewater treatment may contribute to increasing resistance removal efficiency. This study, conducted at a full-scale UWTP, evaluated variations in the bacterial community and ARGs loads and explored possible associations among them. With that aim, the bacterial community composition (16S rRNA gene Illumina sequencing) and ARGs abundance (real-time PCR) were characterized in samples of raw wastewater (RWW), secondary effluent (sTWW), after UV disinfection (tTWW), and after a period of 3 days storage to monitoring possible bacterial regrowth (tTWW-RE). Culturable enterobacteria were also enumerated. Secondary treatment was associated with the most dramatic bacterial community variations and coincided with reductions of ~2 log-units in the ARGs abundance. In contrast, no significant changes in the bacterial community composition and ARGs abundance were observed after UV disinfection of sTWW. Nevertheless, after UV treatment, viability losses were indicated ~2 log-units reductions of culturable enterobacteria. The analysed ARGs (qnrS, blaCTX-M, blaOXA-A, blaTEM, blaSHV, sul1, sul2, and intI1) were strongly correlated with taxa more abundant in RWW than in the other types of water, and which associated with humans and animals, such as members of the families Campylobacteraceae, Comamonadaceae, Aeromonadaceae, Moraxellaceae, and Bacteroidaceae. Further knowledge of the dynamics of the bacterial community during wastewater treatment and its relationship with ARGs variations may contribute with information useful for wastewater treatment optimization, aiming at a more effective resistance control.
AB - Urban wastewater treatment plants (UWTPs) are reservoirs of antibiotic resistance. Wastewater treatment changes the bacterial community and inevitably impacts the fate of antibiotic resistant bacteria and antibiotic resistance genes (ARGs). Some bacterial groups are major carriers of ARGs and hence, their elimination during wastewater treatment may contribute to increasing resistance removal efficiency. This study, conducted at a full-scale UWTP, evaluated variations in the bacterial community and ARGs loads and explored possible associations among them. With that aim, the bacterial community composition (16S rRNA gene Illumina sequencing) and ARGs abundance (real-time PCR) were characterized in samples of raw wastewater (RWW), secondary effluent (sTWW), after UV disinfection (tTWW), and after a period of 3 days storage to monitoring possible bacterial regrowth (tTWW-RE). Culturable enterobacteria were also enumerated. Secondary treatment was associated with the most dramatic bacterial community variations and coincided with reductions of ~2 log-units in the ARGs abundance. In contrast, no significant changes in the bacterial community composition and ARGs abundance were observed after UV disinfection of sTWW. Nevertheless, after UV treatment, viability losses were indicated ~2 log-units reductions of culturable enterobacteria. The analysed ARGs (qnrS, blaCTX-M, blaOXA-A, blaTEM, blaSHV, sul1, sul2, and intI1) were strongly correlated with taxa more abundant in RWW than in the other types of water, and which associated with humans and animals, such as members of the families Campylobacteraceae, Comamonadaceae, Aeromonadaceae, Moraxellaceae, and Bacteroidaceae. Further knowledge of the dynamics of the bacterial community during wastewater treatment and its relationship with ARGs variations may contribute with information useful for wastewater treatment optimization, aiming at a more effective resistance control.
KW - Antibiotic resistance genes
KW - Bacterial community dynamics
KW - Network analysis
KW - Wastewater treatment
UR - http://www.scopus.com/inward/record.url?scp=85047923292&partnerID=8YFLogxK
U2 - 10.1016/j.envint.2018.05.040
DO - 10.1016/j.envint.2018.05.040
M3 - Article
C2 - 29883764
AN - SCOPUS:85047923292
SN - 0160-4120
VL - 118
SP - 179
EP - 188
JO - Environment International
JF - Environment International
ER -
