Bioactive compounds and scavenging capacity of extracts from different parts of Vismia cauliflora against reactive oxygen and nitrogen species

Alessandra Braga Ribeiro, Alessandra Berto, Renan Campos Chisté*, Marisa Freitas, Jesuí V. Visentainer, Eduarda Fernandes

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Context: Vismia cauliflora A.C.Sm. [Hypericaceae (Clusiaceae)] is a plant from Amazonian forest. It is used by Amerindians to treat dermatosis and inflammatory processes in the skin and has been considered an interesting source of bioactive compounds. Objective: We evaluated the scavenging capacity of extracts from V. cauliflora (leaf, branch, stem bark, flower, and whole fruit) against reactive oxygen (ROS) and nitrogen species (RNS), namely, superoxide radical (), hydrogen peroxide (H2O2), hypochlorous acid (HOCl), singlet oxygen (1O2), nitric oxide (NO), and peroxynitrite (ONOO-). In addition, for the first time, the profile of phenolic compounds and carotenoids was determined. Materials and methods: The scavenging capacities of each extract were determined using specific probes (fluorescent, colorimetric, and chemiluminescent) to detect different reactive species (1O2, HOCl, H2O2, NO, and ONOO-). The identification and the quantification of phenolic compounds and carotenoids were carried out by HPLC-DAD-ESI-MS/MS and HPLC-DAD, respectively. Results: (-)-Epicatechin and proanthocyanidin dimers and trimer were the major phenolic compounds tentatively identified in leaf, branch, stem bark, and flower extracts, while dihydroxybenzoic acids were the major compounds in whole fruit extracts. All-trans-zeinoxanthin and all-trans-β-carotene were the major carotenoids tentatively identified in leaf extracts. All extracts of V. cauliflora showed high efficiency against all tested ROS and RNS, although flower and stem bark extracts exhibited the most remarkable scavenging capacity, especially for NO and ONOO-. Discussion and conclusion: Vismia cauliflora has great potential to be used in the development of phytopharmaceutical products due to its characteristic of being a promising source of bioactive compounds with high antioxidant properties.
Original languageEnglish
Pages (from-to)1267-1276
Number of pages10
JournalPharmaceutical Biology
Volume53
Issue number9
DOIs
Publication statusPublished - 2015
Externally publishedYes

Keywords

  • Amazonian plant
  • Antioxidant capacity
  • Carotenoids
  • LC-MS
  • Phenolic compounds

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