Bioactive macro/micro porous silk fibroin/nano-sized calcium phosphate scaffolds with potential for bone-tissue-engineering applications

Le Ping Yan, Joana Silva-Correia, Cristina Correia, Sofia G. Caridade, Emanuel M. Fernandes, Rui A. Sousa, João F. Mano, Joaquim M. Oliveira, Ana L. Oliveira, Rui L. Reis*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)

Abstract

Aim: The development of novel silk/nano-sized calcium phosphate (silk/nano-CaP) scaffolds with highly dispersed CaP nanoparticles in the silk fibroin (SF) matrix for bone tissue engineering. Materials & methods: Nano-CaP was incorporated in a concentrated aqueous SF solution (16 wt.%) by using an in situ synthesis method. The silk/nano-CaP scaffolds were then prepared through a combination of salt-leaching/lyophilization approaches. Results: The CaP particles presented good affinity to SF and their size was inferior to 200 nm when theoretical CaP/silk ratios were between 4 and 16 wt.%, as determined by scanning electron microscopy. The CaP particles displayed a uniform distribution in the scaffolds at both microscopic and macroscopic scales as observed by backscattered scanning electron microscopy and micro-computed tomography, respectively. The prepared scaffolds presented self-mineralization capability and no cytotoxicity confirmed by in vitro bioactivity tests and cell viability assays, respectively. Conclusion: These results indicated that the produced silk/nano-CaP scaffolds could be suitable candidates for bone-tissue-engineering applications.

Original languageEnglish
Pages (from-to)359-378
Number of pages20
JournalNanomedicine
Volume8
Issue number3
DOIs
Publication statusPublished - Mar 2013

Keywords

  • Bone tissue engineering
  • Nano-sized calcium phosphate
  • Nanocomposite
  • Porous scaffold
  • Silk fibroin

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