Bioactivity of novel functionally structured titanium-ceramic composites in contact with human osteoblasts

Gabriella M. Peñarrieta-Juanito; Mafalda Costa; Mariana Cruz; Georgina Miranda; Bruno Henriques; Joana Marques; Ricardo Magini; Antonio Mata; João Caramês; Filipe Silva; Júlio C.M. Souza

doi:10.1002/jbm.a.36394

Bioactivity of novel functionally structured titanium-ceramic composites in contact with human osteoblasts

Gabriella M. Peñarrieta-Juanito, Mafalda Costa, Mariana Cruz, Georgina Miranda, Bruno Henriques, Joana Marques, Ricardo Magini, Antonio Mata, João Caramês, Filipe Silva, Júlio C.M. Souza^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The aim of this study was to analyze the osteogenic cell behavior on the surface of novel functionally graded titanium-based composites containing bioactive ceramics. Titanium grade V discs (8 × 3 mm) embedding gradual content of hydroxyapatite (TiAlV-HA) or beta-tricalcium phosphate (TiAlV-βTCP) were produced by hot-pressing technique. Titanium-ceramic composite discs and Ti grade V (control group) were placed in contact with human osteoblast culture assays. The morphology and adhesion of osteoblasts were inspected by field emission guns scanning electron microscopy (FEGSEM) while cell viability was assessed by fluorometric method. Alkaline phosphatase (ALP) activity and fluorescent microscopic analyses were used to evaluate mineralization on the test and control discs. FEGSEM images showed cells adhered to Ti6Al4V-ceramic and Ti6Al4V surfaces over a period of 24 h, and therefore, an intense proliferation of osteoblasts and spreading cells was noticed for 7 days. Cell viability increased with time on all the surfaces although TiAlV-βTCP revealed significant higher percentage of cell viability than that recorded for TiAlV-HA (p < 0.01). TiAlV-βTCP also showed the highest hydrophilic character. ALP levels increased on the Ti6Al4V-ceramic surfaces when compared to the control group. Also, a qualitative analysis of mineralization evidenced an increase in mineral content on TiAlV-HA or TiAlV-βTCP groups. Novel functionally graded composites based on Ti grade V and hydroxyapatite or βTCP showed a higher bioactivity in presence of osteoblasts than that recorded on Ti grade V. Also, such functionally graded materials can prevent risks of failures by detachment of bioactive ceramic materials during implant placement.

Original language	English
Pages (from-to)	1923-1931
Number of pages	9
Journal	Journal of Biomedical Materials Research - Part A
Volume	106
Issue number	7
DOIs	https://doi.org/10.1002/jbm.a.36394
Publication status	Published - Jul 2018
Externally published	Yes

Keywords

bioactivity
FGM
functionally graded materials
hydroxyapatite
titanium
βTCP

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10.1002/jbm.a.36394

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Peñarrieta-Juanito, G. M., Costa, M., Cruz, M., Miranda, G., Henriques, B., Marques, J., Magini, R., Mata, A., Caramês, J., Silva, F., & Souza, J. C. M. (2018). Bioactivity of novel functionally structured titanium-ceramic composites in contact with human osteoblasts. Journal of Biomedical Materials Research - Part A, 106(7), 1923-1931. https://doi.org/10.1002/jbm.a.36394

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title = "Bioactivity of novel functionally structured titanium-ceramic composites in contact with human osteoblasts",

abstract = "The aim of this study was to analyze the osteogenic cell behavior on the surface of novel functionally graded titanium-based composites containing bioactive ceramics. Titanium grade V discs (8 × 3 mm) embedding gradual content of hydroxyapatite (TiAlV-HA) or beta-tricalcium phosphate (TiAlV-βTCP) were produced by hot-pressing technique. Titanium-ceramic composite discs and Ti grade V (control group) were placed in contact with human osteoblast culture assays. The morphology and adhesion of osteoblasts were inspected by field emission guns scanning electron microscopy (FEGSEM) while cell viability was assessed by fluorometric method. Alkaline phosphatase (ALP) activity and fluorescent microscopic analyses were used to evaluate mineralization on the test and control discs. FEGSEM images showed cells adhered to Ti6Al4V-ceramic and Ti6Al4V surfaces over a period of 24 h, and therefore, an intense proliferation of osteoblasts and spreading cells was noticed for 7 days. Cell viability increased with time on all the surfaces although TiAlV-βTCP revealed significant higher percentage of cell viability than that recorded for TiAlV-HA (p < 0.01). TiAlV-βTCP also showed the highest hydrophilic character. ALP levels increased on the Ti6Al4V-ceramic surfaces when compared to the control group. Also, a qualitative analysis of mineralization evidenced an increase in mineral content on TiAlV-HA or TiAlV-βTCP groups. Novel functionally graded composites based on Ti grade V and hydroxyapatite or βTCP showed a higher bioactivity in presence of osteoblasts than that recorded on Ti grade V. Also, such functionally graded materials can prevent risks of failures by detachment of bioactive ceramic materials during implant placement.",

keywords = "bioactivity, FGM, functionally graded materials, hydroxyapatite, titanium, βTCP",

author = "Pe{\~n}arrieta-Juanito, {Gabriella M.} and Mafalda Costa and Mariana Cruz and Georgina Miranda and Bruno Henriques and Joana Marques and Ricardo Magini and Antonio Mata and Jo{\~a}o Caram{\^e}s and Filipe Silva and Souza, {J{\'u}lio C.M.}",

note = "Publisher Copyright: {\textcopyright} 2018 Wiley Periodicals, Inc.",

year = "2018",

month = jul,

doi = "10.1002/jbm.a.36394",

language = "English",

volume = "106",

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Peñarrieta-Juanito, GM, Costa, M, Cruz, M, Miranda, G, Henriques, B, Marques, J, Magini, R, Mata, A, Caramês, J, Silva, F & Souza, JCM 2018, 'Bioactivity of novel functionally structured titanium-ceramic composites in contact with human osteoblasts', Journal of Biomedical Materials Research - Part A, vol. 106, no. 7, pp. 1923-1931. https://doi.org/10.1002/jbm.a.36394

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AU - Peñarrieta-Juanito, Gabriella M.

AU - Costa, Mafalda

AU - Cruz, Mariana

AU - Miranda, Georgina

AU - Henriques, Bruno

AU - Marques, Joana

AU - Magini, Ricardo

AU - Mata, Antonio

AU - Caramês, João

AU - Silva, Filipe

AU - Souza, Júlio C.M.

PY - 2018/7

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N2 - The aim of this study was to analyze the osteogenic cell behavior on the surface of novel functionally graded titanium-based composites containing bioactive ceramics. Titanium grade V discs (8 × 3 mm) embedding gradual content of hydroxyapatite (TiAlV-HA) or beta-tricalcium phosphate (TiAlV-βTCP) were produced by hot-pressing technique. Titanium-ceramic composite discs and Ti grade V (control group) were placed in contact with human osteoblast culture assays. The morphology and adhesion of osteoblasts were inspected by field emission guns scanning electron microscopy (FEGSEM) while cell viability was assessed by fluorometric method. Alkaline phosphatase (ALP) activity and fluorescent microscopic analyses were used to evaluate mineralization on the test and control discs. FEGSEM images showed cells adhered to Ti6Al4V-ceramic and Ti6Al4V surfaces over a period of 24 h, and therefore, an intense proliferation of osteoblasts and spreading cells was noticed for 7 days. Cell viability increased with time on all the surfaces although TiAlV-βTCP revealed significant higher percentage of cell viability than that recorded for TiAlV-HA (p < 0.01). TiAlV-βTCP also showed the highest hydrophilic character. ALP levels increased on the Ti6Al4V-ceramic surfaces when compared to the control group. Also, a qualitative analysis of mineralization evidenced an increase in mineral content on TiAlV-HA or TiAlV-βTCP groups. Novel functionally graded composites based on Ti grade V and hydroxyapatite or βTCP showed a higher bioactivity in presence of osteoblasts than that recorded on Ti grade V. Also, such functionally graded materials can prevent risks of failures by detachment of bioactive ceramic materials during implant placement.

AB - The aim of this study was to analyze the osteogenic cell behavior on the surface of novel functionally graded titanium-based composites containing bioactive ceramics. Titanium grade V discs (8 × 3 mm) embedding gradual content of hydroxyapatite (TiAlV-HA) or beta-tricalcium phosphate (TiAlV-βTCP) were produced by hot-pressing technique. Titanium-ceramic composite discs and Ti grade V (control group) were placed in contact with human osteoblast culture assays. The morphology and adhesion of osteoblasts were inspected by field emission guns scanning electron microscopy (FEGSEM) while cell viability was assessed by fluorometric method. Alkaline phosphatase (ALP) activity and fluorescent microscopic analyses were used to evaluate mineralization on the test and control discs. FEGSEM images showed cells adhered to Ti6Al4V-ceramic and Ti6Al4V surfaces over a period of 24 h, and therefore, an intense proliferation of osteoblasts and spreading cells was noticed for 7 days. Cell viability increased with time on all the surfaces although TiAlV-βTCP revealed significant higher percentage of cell viability than that recorded for TiAlV-HA (p < 0.01). TiAlV-βTCP also showed the highest hydrophilic character. ALP levels increased on the Ti6Al4V-ceramic surfaces when compared to the control group. Also, a qualitative analysis of mineralization evidenced an increase in mineral content on TiAlV-HA or TiAlV-βTCP groups. Novel functionally graded composites based on Ti grade V and hydroxyapatite or βTCP showed a higher bioactivity in presence of osteoblasts than that recorded on Ti grade V. Also, such functionally graded materials can prevent risks of failures by detachment of bioactive ceramic materials during implant placement.

KW - bioactivity

KW - FGM

KW - functionally graded materials

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KW - titanium

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JO - Journal of Biomedical Materials Research - Part A

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ER -

Bioactivity of novel functionally structured titanium-ceramic composites in contact with human osteoblasts

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