Biochemical characterization of SFC-1, a class A carbapenem-hydrolyzing β-lactamase

Fátima Fonseca, Ana Cristina Sarmento, Isabel Henriques, Bart Samyn, Jozef Van Beeumen, Pedro Domingues, Maria Rosário Domingues, Maria José Saavedra, António Correia*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

The carbapenem-hydrolyzing β-lactamase SFC-1 from Serratia fonticola UTAD54 was overexpressed in Escherichia coli, purified, and characterized. The enzyme exhibited an apparent molecular mass of 30.5 kDa, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SFC-1 hydrolyzes penicillins, cephalosporins, aztreonam, and carbapenems and is inhibited by clavulanic acid, sulbactam, and tazobactam.
Original languageEnglish
Pages (from-to)4512-4514
Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Volume51
Issue number12
DOIs
Publication statusPublished - Dec 2007
Externally publishedYes

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