TY - GEN
T1 - Biodegradable nanoparticles of alginate and chitosan as non-viral DNA oral delivery system
AU - Goncalves, Lidia M. D.
AU - Cadete, Ana
AU - Figueiredo, Lara
AU - Calado, Cecilia C. R.
AU - Almeida, Antonio J.
PY - 2011
Y1 - 2011
N2 - The delivery of nucleic acids via the oral route involves overcoming barriers such as degradation of nucleic acids by low pH in the stomach, enzymatic degradation by DNases in the gut, crossing the physical barrier imposed by the mucus layer, cellular uptake, intracellular trafficking and nuclear uptake. As an oral drug carrier system chitosan nanoparticles are ideal, being mucoadhesive, interacting with the anionic sialic acid residues in mucin. In this study, plasmid DNA expressing a humanized secreted Gaussia Luciferase as reporter gene was encapsulated in alginate and chitosan nanoparticles, via a mild ionotropic gelation procedure with sodium tripolyphosphate as a counterion. The nanoparticle system here developed shows effective transfection of different human gastric epithelial cell lines with distinct cell differention. That was confirmed by the expression of luciferase in the different tested conditions, particularly the amount of encapsulated pGLuc.
AB - The delivery of nucleic acids via the oral route involves overcoming barriers such as degradation of nucleic acids by low pH in the stomach, enzymatic degradation by DNases in the gut, crossing the physical barrier imposed by the mucus layer, cellular uptake, intracellular trafficking and nuclear uptake. As an oral drug carrier system chitosan nanoparticles are ideal, being mucoadhesive, interacting with the anionic sialic acid residues in mucin. In this study, plasmid DNA expressing a humanized secreted Gaussia Luciferase as reporter gene was encapsulated in alginate and chitosan nanoparticles, via a mild ionotropic gelation procedure with sodium tripolyphosphate as a counterion. The nanoparticle system here developed shows effective transfection of different human gastric epithelial cell lines with distinct cell differention. That was confirmed by the expression of luciferase in the different tested conditions, particularly the amount of encapsulated pGLuc.
KW - Cell
KW - Chitosan
KW - Nanoparticles
KW - Plasmid
KW - Tranfection
UR - http://www.scopus.com/inward/record.url?scp=80053971446&partnerID=8YFLogxK
U2 - 10.1109/ENBENG.2011.6026051
DO - 10.1109/ENBENG.2011.6026051
M3 - Conference contribution
AN - SCOPUS:80053971446
SN - 9781457705229
T3 - 1st Portuguese Meeting in Biomedical Engineering, ENBENG 2011
BT - 1st Portuguese Meeting in Biomedical Engineering, ENBENG 2011
T2 - 2011 1st Portuguese Meeting in Bioengineering: The Challenge of the XXI Century, ENBENG 2011
Y2 - 1 March 2011 through 4 March 2011
ER -