Biodegradation and metabolic pathway of the neonicotinoid insecticide thiamethoxam by Labrys portucalensis F11

Oumeima Boufercha, Ana R. Monforte, Allaoueddine Boudemagh, António C. Ferreira, Paula M. L. Castro, Irina S. Moreira*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Downloads

Abstract

Thiamethoxam (TMX) is an effective neonicotinoid insecticide. However, its widespread use is detrimental to non-targeted organisms and water systems. This study investigates the biodegradation of this insecticide by Labrys portucalensis F11. After 30 days of incubation in mineral salt medium, L. portucalensis F11 was able to remove 41%, 35% and 100% of a supplied amount of TMX (10.8 mg L−1) provided as the sole carbon and nitrogen source, the sole carbon and sulfur source and as the sole carbon source, respectively. Periodic feeding with sodium acetate as the supplementary carbon source resulted in faster degradation of TMX (10.8 mg L−1); more than 90% was removed in 3 days. The detection and identification of biodegradation intermediates was performed by UPLC-QTOF/MS/MS. The chemical structure of 12 metabolites is proposed. Nitro reduction, oxadiazine ring cleavage and dechlorination are the main degradation pathways proposed. After biodegradation, toxicity was removed as indicated using Aliivibrio fischeri and by assessing the synthesis of an inducible β-galactosidase by an E. coli mutant (Toxi-Chromo test). L. portucalensis F11 was able to degrade TMX under different conditions and could be effective in bioremediation strategies.
Original languageEnglish
Article number14326
Number of pages17
JournalInternational Journal of Molecular Sciences
Volume23
Issue number22
DOIs
Publication statusPublished - 18 Nov 2022

Keywords

  • Labrys portucalensis F11
  • Biodegradation
  • Metabolites
  • Thiamethoxam
  • Toxicity

Fingerprint

Dive into the research topics of 'Biodegradation and metabolic pathway of the neonicotinoid insecticide thiamethoxam by Labrys portucalensis F11'. Together they form a unique fingerprint.

Cite this