TY - JOUR
T1 - Biodegradation of sulfamethoxazole and other sulfonamides by Achromobacter denitrificans PR1
AU - Reis, Patrícia J. M.
AU - Reis, Ana C.
AU - Ricken, Benjamin
AU - Kolvenbach, Boris A.
AU - Manaia, Célia M.
AU - Corvini, Philippe F. X.
AU - Nunes, Olga C.
PY - 2014/9/15
Y1 - 2014/9/15
N2 - This study aimed to isolate and characterize a microbial culture able to degrade sulfonamides. Sulfamethoxazole (SMX)-degrading microorganisms were enriched from activated sludge and wastewater. The resultant mixed culture was composed of four bacterial strains, out of which only Achromobacter denitrificans PR1 could degrade SMX. This sulfonamide was used as sole source of carbon, nitrogen and energy with stoichiometric accumulation of 3-amino-5-methylisoxazole. Strain PR1 was able to remove SMX at a rate of 73.6±9.6μmolSMX/gcell dry weighth. This rate more than doubled when a supplement of amino acids or the other members of the mixed culture were added. Besides SMX, strain PR1 was able to degrade other sulfonamides with anti-microbial activity. Other environmental Achromobacter spp. could not degrade SMX, suggesting that this property is not broadly distributed in members of this genus. Further studies are needed to shed additional light on the genetics and enzymology of this process.
AB - This study aimed to isolate and characterize a microbial culture able to degrade sulfonamides. Sulfamethoxazole (SMX)-degrading microorganisms were enriched from activated sludge and wastewater. The resultant mixed culture was composed of four bacterial strains, out of which only Achromobacter denitrificans PR1 could degrade SMX. This sulfonamide was used as sole source of carbon, nitrogen and energy with stoichiometric accumulation of 3-amino-5-methylisoxazole. Strain PR1 was able to remove SMX at a rate of 73.6±9.6μmolSMX/gcell dry weighth. This rate more than doubled when a supplement of amino acids or the other members of the mixed culture were added. Besides SMX, strain PR1 was able to degrade other sulfonamides with anti-microbial activity. Other environmental Achromobacter spp. could not degrade SMX, suggesting that this property is not broadly distributed in members of this genus. Further studies are needed to shed additional light on the genetics and enzymology of this process.
KW - 3-Amino-5-methylisoxazole
KW - Achromobacter
KW - Antibiotic resistance
KW - Pharmaceutical
KW - Xenobiotics
UR - http://www.scopus.com/inward/record.url?scp=84907984539&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2014.08.039
DO - 10.1016/j.jhazmat.2014.08.039
M3 - Article
C2 - 25238191
AN - SCOPUS:84907984539
SN - 0304-3894
VL - 280
SP - 741
EP - 749
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
ER -
