@article{0543cbf305b147e586c9a4f467a8ba10,
title = "Bioengineering of lanthipeptides in Escherichia coli: assessing the specificity of lichenicidin and haloduracin biosynthetic machinery",
abstract = "The lichenicidin and haloduracin biosynthetic machinery specificity was investigated invivo in Escherichia coli. Unlike previous reports using different hosts, it was found that the biosynthetic machineries of lichenicidin and haloduracin are highly specific to their dedicated peptide precursors. Likewise, the substitution of lichenicidin structural genes by chimeras of lichenicidin leader sequences and haloduracin core peptides did not yield mature haloduracin peptides. Despite these restrictions, it was found that the bifunctional enzyme HalT was able to process and export lichenicidin peptides. These findings corroborate the promiscuity of LanT enzymes reported for other lantibiotics, such as nukacin ISK-1 and lacticin 481.",
keywords = "Bacillus, Bacteriocins, Chimeras, Invivo, Lantibiotics",
author = "T. Caetano and J. Barbosa and E. M{\"o}esker and S{\"u}ssmuth, {R. D.} and S. Mendo",
note = "Funding Information: T{\^a}nia Caetano was supported by Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia , POPH , European Union and Medinfar Pharmaceuticals SA grants SFRH/BDE/15559/2005 and SFRH/BPD/77900/2011 . This work was supported by Coli4Lan project (FCOMP-01-0124-FEDER-027569), funded by Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia I.P. (PIDDAC) and by Fundo Europeu de Desenvolvimento Regional – FEDER , through the COMPETE – Programa Operacional Fatores de Competitividade (POFC). This project also supported Joana Barbosa with the BI/UI88/6285/2013 grant. Additionally, the work was also supported by European Funds through COMPETE and by National Funds through the Portuguese Science Foundation (FCT) within project PEst-C/MAR/LA0017/2013 . The work of R.D.S. was supported by the cluster of Excellence “unifying concepts in catalysis” funded by the German Research Council (DFG). Finally, the authors would like to thank you Joanna Krawczyk for the support in chimera's design. Publisher Copyright: {\textcopyright} 2014 Institut Pasteur.",
year = "2014",
doi = "10.1016/j.resmic.2014.07.006",
language = "English",
volume = "165",
pages = "600--604",
journal = "Research in Microbiology",
issn = "0923-2508",
publisher = "Elsevier Masson SAS",
number = "7",
}