@inbook{393f29967c7e49558221db215d42a876,
title = "Bioluminescent models to evaluate the efficiency of light-based antibacterial approaches",
abstract = "The emergence of microbial resistance to antimicrobials among several common pathogenic microbial strains is an increasing problem worldwide. Thus, it is urgent to develop not only new antimicrobial therapeutics to fight microbial infections, but also new effective, rapid, and inexpensive methods to monitor the efficacy of these new therapeutics. Antimicrobial photodynamic therapy (aPDT) and antimicrobial blue light (aBL) therapy are receiving considerable attention for their antimicrobial potential and represent realistic alternatives to antibiotics. To monitor the photoinactivation process provided by aPDT and aBL, faster and more effective methods are required instead of laborious conventional plating and overnight incubation procedures. Bioluminescent microbial models are very interesting in this context. Light emission from bioluminescent microorganisms is a highly sensitive indication of their metabolic activity and can be used to monitor, in real time, the effects of antimicrobial agents and therapeutics. This chapter reviews the efforts of the scientific community concerning the development of in vitro, ex vivo, and in vivo bioluminescent bacterial models and their potential to evaluate the efficiency of aPDT and aBL in the inactivation of bacteria.",
keywords = "Alternative approaches, Antimicrobial blue light therapy (aBL), Antimicrobial photodynamic therapy (aPDT), Bacteria, Bioluminescent models, Biomedical research, Drug monitoring, Photosensitizers, Resistance",
author = "Gomes, {Ana T. P. C.} and Faustino, {Maria A. F.} and Neves, {Maria G. P. M. S.} and Adelaide Almeida",
note = "Funding Information: The authors are grateful to the University of Aveiro and the FCT/MCT for the financial support for the CESAM (UID/50017/2020) and LAQV-REQUIMTE (UIDB/50006/ 2020), and the FCT project PREVINE (FCT-PTDC/ASP-PES/ 29576/2017), through national funds and, where applicable, co-financed by the FEDER. Publisher Copyright: {\textcopyright} 2022, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2099-1_34",
language = "English",
isbn = "9781071620984",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "631--669",
editor = "Mans Broekgaarden and Hong Zhang and Mladen Korbelik and Hamblin, {Michael R.} and Michal Heger",
booktitle = "Methods in molecular biology",
edition = "1",
}