TY - JOUR
T1 - Biopolymer from water kefir as a potential clean-label ingredient for health applications
T2 - evaluation of new properties
AU - Lucena, Monalisa de Alencar
AU - Ramos, Igor Frederico da Silveira
AU - Geronço, Maurycyo Silva
AU - Araújo, Ricardo de
AU - Filho, Francisco Lopes da Silva
AU - Silva, Luís Manuel Lopes Rodrigues da
AU - Sousa, Rayran Walter Ramos de
AU - Ferreira, Paulo Michel Pinheiro
AU - Osajima, Josy Anteveli
AU - Silva-Filho, Edson Cavalcanti
AU - Rizzo, Márcia dos Santos
AU - Ribeiro, Alessandra Braga
AU - Costa, Marcilia Pinheiro da
PY - 2022/6/17
Y1 - 2022/6/17
N2 - The present work aimed to characterize the exopolysaccharide obtained from water kefir grains (EPSwk), a symbiotic association of probiotic microorganisms. New findings of the technological, mechanical, and biological properties of the sample were studied. The EPSwk polymer presented an Mw of 6.35 × 105 Da. The biopolymer also showed microcrystalline structure and characteristic thermal stability with maximum thermal degradation at 250 °C. The analysis of the monosaccharides of the EPSwk by gas chromatography demonstrated that the material is composed of glucose units (98 mol%). Additionally, EPSwk exhibited excellent emulsifying properties, film-forming ability, a low photodegradation rate (3.8%), and good mucoadhesive properties (adhesion Fmax of 1.065 N). EPSwk presented cytocompatibility and antibacterial activity against Escherichia coli and Staphylococcus aureus. The results of this study expand the potential application of the exopolysaccharide from water kefir as a potential clean-label raw material for pharmaceutical, biomedical, and cosmetic applications.
AB - The present work aimed to characterize the exopolysaccharide obtained from water kefir grains (EPSwk), a symbiotic association of probiotic microorganisms. New findings of the technological, mechanical, and biological properties of the sample were studied. The EPSwk polymer presented an Mw of 6.35 × 105 Da. The biopolymer also showed microcrystalline structure and characteristic thermal stability with maximum thermal degradation at 250 °C. The analysis of the monosaccharides of the EPSwk by gas chromatography demonstrated that the material is composed of glucose units (98 mol%). Additionally, EPSwk exhibited excellent emulsifying properties, film-forming ability, a low photodegradation rate (3.8%), and good mucoadhesive properties (adhesion Fmax of 1.065 N). EPSwk presented cytocompatibility and antibacterial activity against Escherichia coli and Staphylococcus aureus. The results of this study expand the potential application of the exopolysaccharide from water kefir as a potential clean-label raw material for pharmaceutical, biomedical, and cosmetic applications.
KW - Antimicrobial activity
KW - Biopolymer
KW - Dextran
KW - Mucoadhesiveness
KW - Photostability
KW - Polysaccharide
UR - http://www.scopus.com/inward/record.url?scp=85132933767&partnerID=8YFLogxK
U2 - 10.3390/molecules27123895
DO - 10.3390/molecules27123895
M3 - Article
C2 - 35745016
SN - 1420-3049
VL - 27
JO - Molecules
JF - Molecules
IS - 12
M1 - 3895
ER -