TY - JOUR
T1 - Bioprospecting antibiotic properties in photodynamic therapy of lipids from Codium tomemtosum and Chlorella vulgaris
AU - Bartolomeu, Maria
AU - Vieira, Cátia
AU - Dias, Marina
AU - Conde, Tiago
AU - Couto, Daniela
AU - Lopes, Diana
AU - Neves, Bruna
AU - Melo, Tânia
AU - Rey, Felisa
AU - Alves, Eliana
AU - Silva, Joana
AU - Abreu, Helena
AU - Almeida, Adelaide
AU - Domingues, M. Rosário
N1 - Funding Information:
This research was funded by FCT / MCT through the financial support of LAQV-REQUIMTE ( UIDB/50006/2020 + UIDB/50006/2020 ) and CESAM ( UID/AMB/50017/2019 + UIDP/50017/2020 + LA/P/0094/2020 ) research units and project AlgaValor, from the Portugal 2020 program (grant agreement nº POCI-01-0247-FEDER-035234 ; LISBOA-01-0247-FEDER-035234 ; ALG-01-0247-FEDER-035234 ).
Funding Information:
The authors would like to thank the University of Aveiro , the Department of Biology, and the Department of Chemistry, where the experiments were performed; to the Fundação para a Ciência e a Tecnologia (FCT) for the LAQV-REQUIMTE the LAQV-REQUIMTE (UIDP/50006/2020 + UIDB/50006/2020), and CESAM (UIDB/50017/2020 + UIDP/50017/2020 + LA/P/0094/2020) and Portuguese Mass Spectrometry Network (RNEM, LISBOA-01-0145-FEDER-402-022125) funding. Maria Bartolomeu (SFRH/BD/121645/2016), Cátia Vieira (SFRH/BD/150358/2019), Daniela Salomé Moderno do Couto (SFRH/BD/138992/2018), Bruna Neves (2021.04602.BD) and Tiago Alexandre Conde (2020.05678.BD) are grateful to FCT for their PhD grants. Tania Melo, Felisa Rey, and Eliana Alves thanks the Junior Researcher contract in the scope of the Individual Call to Scientific Employment Stimulus [CEECIND/01578/2020, Tânia Melo, CEECIND/00580/2017, Felisa Rey and CEECIND/00971/2017, Eliana Alves]. This work has received funding under the project AlgaValor, from the Portugal 2020 program (grant agreement nº POCI-01-0247-FEDER-035234 ; LISBOA-01-0247-FEDER-035234 ; ALG-01-0247-FEDER-035234 ). The authors are also thankful to the COST Action EpiLipidNET, CA19105 - Pan-European Network in Lipidomics and EpiLipidomics. This work is a contribution of the Marine Lipidomics laboratory.
Publisher Copyright:
© 2022 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM)
PY - 2022/12
Y1 - 2022/12
N2 - The growing resistance from pathogens against antibiotics has increased the research for new compounds and strategies with antibacterial potential. Lipids from algae are emerging as natural and potent bioactive molecules with interesting results regarding the inactivation of bacteria, viruses, and fungi. The combination of algae lipids with innovative strategies, such as antibacterial photodynamic therapy (aPDT) can enhance their antimicrobial potential. In this work, we aimed to evaluate the antibacterial potential in aPDT of total lipid extracts and polar lipid fractions from the green macroalga, Codium tomentosum, and the green microalga, Chlorella vulgaris on a Gram-positive bacteria Staphylococcus aureus. Total lipid extracts and polar lipid fractions were characterized by LC-MS. The results revealed that the total extracts of algae promote S. aureus inhibition after light irradiation, with a decrease of ca. 6 log10 (CFU/mL) after 15 min of treatment with both extracts of algae. The polar lipid fractions, composed by phospholipids, glycolipids and betaine lipids, from C. tomentosum and C. vulgaris also revealed antibacterial potential in combination with aPDT, but a decrease of ca. 6 log10 (CFU/mL) was reached at 60 min of treatment, later than with the total extracts. These results unveil algae lipids as antibacterial compounds in combination with aPDT displaying an alternative from natural origin to tackle pathogen resistance.
AB - The growing resistance from pathogens against antibiotics has increased the research for new compounds and strategies with antibacterial potential. Lipids from algae are emerging as natural and potent bioactive molecules with interesting results regarding the inactivation of bacteria, viruses, and fungi. The combination of algae lipids with innovative strategies, such as antibacterial photodynamic therapy (aPDT) can enhance their antimicrobial potential. In this work, we aimed to evaluate the antibacterial potential in aPDT of total lipid extracts and polar lipid fractions from the green macroalga, Codium tomentosum, and the green microalga, Chlorella vulgaris on a Gram-positive bacteria Staphylococcus aureus. Total lipid extracts and polar lipid fractions were characterized by LC-MS. The results revealed that the total extracts of algae promote S. aureus inhibition after light irradiation, with a decrease of ca. 6 log10 (CFU/mL) after 15 min of treatment with both extracts of algae. The polar lipid fractions, composed by phospholipids, glycolipids and betaine lipids, from C. tomentosum and C. vulgaris also revealed antibacterial potential in combination with aPDT, but a decrease of ca. 6 log10 (CFU/mL) was reached at 60 min of treatment, later than with the total extracts. These results unveil algae lipids as antibacterial compounds in combination with aPDT displaying an alternative from natural origin to tackle pathogen resistance.
KW - Antibacterial
KW - Lipidomics
KW - Microalgae
KW - Photodynamic therapy
KW - Polar lipids
KW - Seaweeds
UR - http://www.scopus.com/inward/record.url?scp=85139084675&partnerID=8YFLogxK
U2 - 10.1016/j.biochi.2022.09.012
DO - 10.1016/j.biochi.2022.09.012
M3 - Article
C2 - 36181961
AN - SCOPUS:85139084675
SN - 0300-9084
VL - 203
SP - 32
EP - 39
JO - Biochimie
JF - Biochimie
ER -