TY - JOUR
T1 - Brassica oleracea L. Var. costata DC and Pieris brassicae L. Aqueous extracts reduce methyl methanesulfonate-induced DNA damage in V79 hamster lung fibroblasts
AU - Sousa, Carla
AU - Fernandes, Fátima
AU - Valentão, Patrícia
AU - Rodrigues, António Sebastião
AU - Coelho, Marta
AU - Teixeira, João P.
AU - Silva, Susana
AU - Ferreres, Federico
AU - Pinho, Paula Guedes de
AU - Andrade, Paula B.
PY - 2012/5/30
Y1 - 2012/5/30
N2 - Brassica oleracea L. var. costata DC leaves and Pieris brassicae L. larvae aqueous extracts were assayed for their potential to prevent/induce DNA damage. None of them was mutagenic at the tested concentrations in the Ames test reversion assay using Salmonella His+ TA98 strains, with and without metabolic activation. In the hypoxanthine-guanine phosphoribosyltransferase mutation assay using mammalian V79 fibroblast cell line, extracts at 500 μg/mL neither induced mutations nor protected against the mutagenicity caused by methyl methanesulfonate (MMS). In the comet assay, none of the extracts revealed to be genotoxic by itself, and both afforded protection, more pronounced for larvae extracts, against MMS-induced genotoxicity. As genotoxic/antigenotoxic effects of Brassica vegetables are commonly attributed to isothiocyanates, the extracts were screened for these compounds by headspace-solid-phase microextraction/gas chromatography-mass spectrometry. No sulfur compound was detected. These findings demonstrate that both extracts could be useful against damage caused by genotoxic compounds, the larvae extract being the most promising.
AB - Brassica oleracea L. var. costata DC leaves and Pieris brassicae L. larvae aqueous extracts were assayed for their potential to prevent/induce DNA damage. None of them was mutagenic at the tested concentrations in the Ames test reversion assay using Salmonella His+ TA98 strains, with and without metabolic activation. In the hypoxanthine-guanine phosphoribosyltransferase mutation assay using mammalian V79 fibroblast cell line, extracts at 500 μg/mL neither induced mutations nor protected against the mutagenicity caused by methyl methanesulfonate (MMS). In the comet assay, none of the extracts revealed to be genotoxic by itself, and both afforded protection, more pronounced for larvae extracts, against MMS-induced genotoxicity. As genotoxic/antigenotoxic effects of Brassica vegetables are commonly attributed to isothiocyanates, the extracts were screened for these compounds by headspace-solid-phase microextraction/gas chromatography-mass spectrometry. No sulfur compound was detected. These findings demonstrate that both extracts could be useful against damage caused by genotoxic compounds, the larvae extract being the most promising.
KW - Brassica oleracea var. costata
KW - Genoprotection
KW - Pieris brassicae larvae
UR - http://www.scopus.com/inward/record.url?scp=84861592201&partnerID=8YFLogxK
U2 - 10.1021/jf300941s
DO - 10.1021/jf300941s
M3 - Article
C2 - 22582708
AN - SCOPUS:84861592201
SN - 0021-8561
VL - 60
SP - 5380
EP - 5387
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 21
ER -