TY - JOUR
T1 - Cell-based in vitro models for predicting drug permeability
AU - Sarmento, Bruno
AU - Andrade, Fernanda
AU - Silva, Sara Baptista da
AU - Rodrigues, Francisca
AU - Das Neves, José
AU - Ferreira, Domingos
PY - 2012/5
Y1 - 2012/5
N2 - In vitro cell models have been used to predict drug permeation in early stages of drug development, since they represent an easy and reproducible method, allowing the tracking of drug absorption rate and mechanism, with an advantageous costbenefit ratio. Such cell-based models are mainly composed of immortalized cells with an intrinsic ability to grow in a monolayer when seeded in permeable supports, maintaining their physiologic characteristics regarding epithelium cell physiology and functionality. Areas covered: This review summarizes the most important intestinal, pulmonary, nasal, vaginal, rectal, ocular and skin cell-based in vitro models for predicting the permeability of drugs. Moreover, the similitude between in vitro cell models and in vivo conditions are discussed, providing evidence that each model may provisionally resemble different drug absorption route. Expert opinion: Despite the widespread use of in vitro cell models for drug permeability and absorption evaluation purposes, a detailed study on the properties of these models and their in vitroin vivo correlation compared with human data are required to further use in order to consider a future drug discovery optimization and clinical development.
AB - In vitro cell models have been used to predict drug permeation in early stages of drug development, since they represent an easy and reproducible method, allowing the tracking of drug absorption rate and mechanism, with an advantageous costbenefit ratio. Such cell-based models are mainly composed of immortalized cells with an intrinsic ability to grow in a monolayer when seeded in permeable supports, maintaining their physiologic characteristics regarding epithelium cell physiology and functionality. Areas covered: This review summarizes the most important intestinal, pulmonary, nasal, vaginal, rectal, ocular and skin cell-based in vitro models for predicting the permeability of drugs. Moreover, the similitude between in vitro cell models and in vivo conditions are discussed, providing evidence that each model may provisionally resemble different drug absorption route. Expert opinion: Despite the widespread use of in vitro cell models for drug permeability and absorption evaluation purposes, a detailed study on the properties of these models and their in vitroin vivo correlation compared with human data are required to further use in order to consider a future drug discovery optimization and clinical development.
KW - Cell models
KW - In vitro models
KW - In vitro-in vivo correlation
KW - Intestinal mucosa
KW - Permeability
UR - http://www.scopus.com/inward/record.url?scp=84859967380&partnerID=8YFLogxK
U2 - 10.1517/17425255.2012.673586
DO - 10.1517/17425255.2012.673586
M3 - Review article
C2 - 22424145
AN - SCOPUS:84859967380
SN - 1742-5255
VL - 8
SP - 607
EP - 621
JO - Expert Opinion on Drug Metabolism and Toxicology
JF - Expert Opinion on Drug Metabolism and Toxicology
IS - 5
ER -