@article{bb4fb1ae4db54da3ae064b51f4085ca3,
title = "Cell-type-specific TEV protease cleavage reveals cohesin functions in drosophila neurons",
abstract = "Cohesin is a highly conserved multisubunit complex that holds sister chromatids together in mitotic cells. At the metaphase to anaphase transition, proteolytic cleavage of the α kleisin subunit (Rad21) by separase causes cohesin's dissociation from chromosomes and triggers sister-chromatid disjunction. To investigate cohesin's function in postmitotic cells, where it is widely expressed, we have created fruit flies whose Rad21 can be cleaved by TEV protease. Cleavage causes precocious separation of sister chromatids and massive chromosome missegregation in proliferating cells, but not disaggregation of polytene chromosomes in salivary glands. Crucially, cleavage in postmitotic neurons is lethal. In mushroom-body neurons, it causes defects in axon pruning, whereas in cholinergic neurons it causes highly abnormal larval locomotion. These data demonstrate essential roles for cohesin in nondividing cells and also introduce a powerful tool by which to investigate protein function in metazoa.",
keywords = "Cellcycle, Devbio, Molneuro",
author = "Andrea Pauli and Friederike Althoff and Oliveira, {Raquel A.} and Stefan Heidmann and Oren Schuldiner and Lehner, {Christian F.} and Dickson, {Barry J.} and Kim Nasmyth",
note = "Funding Information: We thank L. Luo, G. Miesenboeck, J. Simpson, F. Schnorrer, D. Dorsett, C. Sunkel, R. Paro, J. Lis, P. Harte, J. Zhou, H. Saumweber, U. Laemmli, G. Struhl, K. Keleman, S. Gruber, Gene Exel Korea, and the Bloomington Stock Center for reagents. We are especially grateful to Liqun Luo for sharing unpublished data and for comments and discussion on the manuscript. We also thank H. White-Cooper for providing fly facilities in Oxford and for discussions and comments; A. Graf, K. Jaeger, and R. Fuchs for help with the generation of transgenic flies; and G. Miesenboeck, I. Davis, A. Brand, and all members of the K.N. lab for helpful discussions and comments. Work in the laboratory of K.N. and B.J.D. was supported by funds from Boehringer Ingelheim GmbH (IMP) and grants from the Medical Research Council (MRC) and Wellcome Trust. Work in the laboratories of C.F.L. and S.H. was supported by grants from the Deutsche Forschungsgemeinschaft (DFG Le987/3-4 and He 2354/2-3). ",
year = "2008",
month = feb,
day = "12",
doi = "10.1016/j.devcel.2007.12.009",
language = "English",
volume = "14",
pages = "239--251",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "2",
}