TY - JOUR
T1 - Changes in the salivary proteome of beagle dogs after weight loss
AU - Lucena, S.
AU - Coelho, A. V.
AU - Muñoz-Prieto, A.
AU - Anjo, S. I.
AU - Manadas, B.
AU - Capela e Silva, F.
AU - Lamy, E.
AU - Tvarijonaviciute, A.
N1 - Funding Information:
This paper received national funding through the Portuguese Science Foundation (FCT) under project UIDB/05183/2020. This work was also financed by the European Regional Development Fund through the COMPETE 2020 – Operational Programme for Competitiveness and Internationalisation and Portuguese national funds via FCT, under projects: POCI-01–0145-FEDER-029311; POCI-01–0145-FEDER-007440 (ref. UID/NEU/04539/2013), POCI-01–0145-FEDER-016428 (ref. SAICTPAC/0010/2015) and POCI-01–0145-FEDER-016795 (ref. PTDC/NEU-SCC/7051/2014), and by The National Mass Spectrometry Network (RNEM) under the contract POCI-01–0145-FEDER-402–022125 (ref. ROTEIRO/0028/2013). Financial support was also provided by a grant from the Robles Chillida Foundation through its support program for the promotion of research in Health Sciences. The authors also acknowledge financial support from FCT in the form of Elsa Lamy's FCT investigator contract CEECIND/04397/2017. Asta Tvarijonaviciute was supported by the program “Ramon y Cajal” of the “Ministerio de Economia y Competitividad,” Spain, through a postdoctoral grant. The University of Murcia funded Alberto Muñoz-Prieto through a predoctoral grant. Funders had no role in the study design, data collection, analysis, interpretation, or writing of the report. The authors declare that they have no conflicts of interest.
Publisher Copyright:
© 2020 Elsevier Inc.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/7
Y1 - 2020/7
N2 - Being overweight or obese represents an important health issue in humans and pets. The aim of this study was to investigate changes in the salivary proteome of overweight beagles after induced weight loss to better understand the physiological changes involved in this process. Five overweight/obese neutered males of pure breed beagles were evaluated. During the 3-mo period of weight loss, each animal received a strictly controlled amount of a low fat commercial diet per day. Body condition scores (BCS), body weight (BW), and serum biochemical parameters (total cholesterol, triglycerides, and C-reactive protein) were assessed weekly. Quantitative proteomics analysis by SWATH was used to evaluate the salivary proteome changes induced by weight loss treatment. BCS, BW, serum total cholesterol concentration, and abundances of 23 salivary proteins differed significantly between before and after treatment. Some of the altered protein amounts, namely of peptidyl-prolyl cis-trans isomerase, fructose-bisphosphate aldolase C, and 78-kDa glucose-regulated protein, increased after weight loss. These proteins are related with the immune system, inflammatory status, oxidative stress, and glucose metabolism. The results obtained suggest a potential use of salivary proteins in monitoring physiological changes in dogs subjected to weight loss. Moreover, the type of changes identified reinforces the postulated physiological improvements, which weight loss induces. Further research is needed to determine whether the changes observed in this study are due to weight loss, dietary changes, or a combination of both.
AB - Being overweight or obese represents an important health issue in humans and pets. The aim of this study was to investigate changes in the salivary proteome of overweight beagles after induced weight loss to better understand the physiological changes involved in this process. Five overweight/obese neutered males of pure breed beagles were evaluated. During the 3-mo period of weight loss, each animal received a strictly controlled amount of a low fat commercial diet per day. Body condition scores (BCS), body weight (BW), and serum biochemical parameters (total cholesterol, triglycerides, and C-reactive protein) were assessed weekly. Quantitative proteomics analysis by SWATH was used to evaluate the salivary proteome changes induced by weight loss treatment. BCS, BW, serum total cholesterol concentration, and abundances of 23 salivary proteins differed significantly between before and after treatment. Some of the altered protein amounts, namely of peptidyl-prolyl cis-trans isomerase, fructose-bisphosphate aldolase C, and 78-kDa glucose-regulated protein, increased after weight loss. These proteins are related with the immune system, inflammatory status, oxidative stress, and glucose metabolism. The results obtained suggest a potential use of salivary proteins in monitoring physiological changes in dogs subjected to weight loss. Moreover, the type of changes identified reinforces the postulated physiological improvements, which weight loss induces. Further research is needed to determine whether the changes observed in this study are due to weight loss, dietary changes, or a combination of both.
KW - Dog
KW - Overweight
KW - Proteome
KW - Saliva
KW - Weight loss
UR - http://www.scopus.com/inward/record.url?scp=85083877714&partnerID=8YFLogxK
U2 - 10.1016/j.domaniend.2020.106474
DO - 10.1016/j.domaniend.2020.106474
M3 - Article
C2 - 32361424
AN - SCOPUS:85083877714
SN - 0739-7240
VL - 72
JO - Domestic Animal Endocrinology
JF - Domestic Animal Endocrinology
M1 - 106474
ER -