Changes of antibiotic resistance phenotype in outbreak-linked Salmonella enterica strains after exposure to human simulated gastrointestinal conditions in chicken meat

Camila Veríssimo De Sales, Adma Nadja Ferreira De Melo, Katarzyna Maria Niedzwiedzka, Evandro Leite De Souza, Donald W. Schaffner, Marciane Magnani*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Fifteen outbreak-linked Salmonella enterica strains in chicken meat were evaluated under simulated human gastrointestinal conditions for their resistance and susceptibility to 11 antibiotics from seven antibiotic classes. The MIC of each antibiotic was determined by microdilution in broth before and after the exposure of each strain to a continuous system simulating the conditions in the human mouth, esophagus-stomach, duodenum, and ileum. Strains were inoculated onto chicken breast (9 g; inoculated at 5 log CFU/g) prior to exposure. Data were interpreted according Clinical and Laboratory Standards Institute breakpoints. After the in vitro digestion, 12 Salmonella strains with reduced susceptibility to ciprofloxacin (CIP) changed to CIP resistant. The ceftriaxone (CTX)–intermediate Salmonella Newport strain changed to CTX resistant. The ampicillin (AMP)–susceptible Salmonella Heidelberg strain changed to AMP resistant, and the sulfamethoxazole-trimethoprim (SXT)–susceptible strains of Salmonella serovars Typhimurium, Agona, Newport, Albany, and Corvallis changed to SXT resistant. The Salmonella Heidelberg, Salmonella Newport, Salmonella Albany, and Salmonella Corvallis strains had the highest frequency of changes in antibiotic susceptibility with new resistant phenotypes to AMP and CIP, CTX and SXT, CIP and SXT, and CIP and SXT, respectively. Conditions imposed by a simulated gastrointestinal environment changed the susceptibility of S. enterica strains to clinically relevant antibiotics and should be considered in the selection of therapies for human salmonellosis.

Original languageEnglish
Pages (from-to)1844-1850
Number of pages7
JournalJournal of Food Protection
Volume81
Issue number11
DOIs
Publication statusPublished - Nov 2018
Externally publishedYes

Keywords

  • Epidemic strains
  • In vitro digestion
  • Poultry meat
  • Salmonellosis

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