Characterization of the pJB12 Plasmid from Pseudomonas aeruginosa reveals Tn6352, a novel putative transposon associated with mobilization of the blaVIM-2-harboring In58 integron

João Botelho; Filipa Grosso; Luísa Peixe

doi:10.1128/aac.02532-16

Characterization of the pJB12 Plasmid from Pseudomonas aeruginosa reveals Tn6352, a novel putative transposon associated with mobilization of the blaVIM-2-harboring In58 integron

João Botelho
, Filipa Grosso
, Luísa Peixe^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The blaVIM-2-carrying In58 integron has been linked to a chromosomal location in different bacterial species, including Pseudomonas aeruginosa. This work reports the first fully sequenced In58-harboring plasmid, which is significantly different from the two previously identified blaVIM-2-carrying plasmids in P. aeruginosa. blaVIM-2 might have been acquired by transposition of Tn6352, a novel transposon composed of the In58 and ISPa17 elements. The recognition of similar inverted repeat (IR) sites by ISPa17 reveals a common mobilization process associated with acquisition of the blaVIM-2 and blaVIM-1 genes.

Original language	English
Article number	e02532-16
Pages (from-to)	1-6
Number of pages	6
Journal	Antimicrobial Agents and Chemotherapy
Volume	61
Issue number	5
DOIs	https://doi.org/10.1128/aac.02532-16
Publication status	Published - May 2017

Keywords

Pseudomonas aeruginosa
Carbapenemase
Plasmid-mediated resistance
Transposon

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10.1128/aac.02532-16

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title = "Characterization of the pJB12 Plasmid from Pseudomonas aeruginosa reveals Tn6352, a novel putative transposon associated with mobilization of the blaVIM-2-harboring In58 integron",

abstract = "The blaVIM-2-carrying In58 integron has been linked to a chromosomal location in different bacterial species, including Pseudomonas aeruginosa. This work reports the first fully sequenced In58-harboring plasmid, which is significantly different from the two previously identified blaVIM-2-carrying plasmids in P. aeruginosa. blaVIM-2 might have been acquired by transposition of Tn6352, a novel transposon composed of the In58 and ISPa17 elements. The recognition of similar inverted repeat (IR) sites by ISPa17 reveals a common mobilization process associated with acquisition of the blaVIM-2 and blaVIM-1 genes.",

keywords = "Pseudomonas aeruginosa, Carbapenemase, Plasmid-mediated resistance, Transposon",

author = "Jo{\~a}o Botelho and Filipa Grosso and Lu{\'i}sa Peixe",

year = "2017",

month = may,

doi = "10.1128/aac.02532-16",

language = "English",

volume = "61",

pages = "1--6",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

number = "5",

}

Characterization of the pJB12 Plasmid from Pseudomonas aeruginosa reveals Tn6352, a novel putative transposon associated with mobilization of the blaVIM-2-harboring In58 integron. / Botelho, João; Grosso, Filipa; Peixe, Luísa.
In: Antimicrobial Agents and Chemotherapy, Vol. 61, No. 5, e02532-16, 05.2017, p. 1-6.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Characterization of the pJB12 Plasmid from Pseudomonas aeruginosa reveals Tn6352, a novel putative transposon associated with mobilization of the blaVIM-2-harboring In58 integron

AU - Botelho, João

AU - Grosso, Filipa

AU - Peixe, Luísa

PY - 2017/5

Y1 - 2017/5

N2 - The blaVIM-2-carrying In58 integron has been linked to a chromosomal location in different bacterial species, including Pseudomonas aeruginosa. This work reports the first fully sequenced In58-harboring plasmid, which is significantly different from the two previously identified blaVIM-2-carrying plasmids in P. aeruginosa. blaVIM-2 might have been acquired by transposition of Tn6352, a novel transposon composed of the In58 and ISPa17 elements. The recognition of similar inverted repeat (IR) sites by ISPa17 reveals a common mobilization process associated with acquisition of the blaVIM-2 and blaVIM-1 genes.

AB - The blaVIM-2-carrying In58 integron has been linked to a chromosomal location in different bacterial species, including Pseudomonas aeruginosa. This work reports the first fully sequenced In58-harboring plasmid, which is significantly different from the two previously identified blaVIM-2-carrying plasmids in P. aeruginosa. blaVIM-2 might have been acquired by transposition of Tn6352, a novel transposon composed of the In58 and ISPa17 elements. The recognition of similar inverted repeat (IR) sites by ISPa17 reveals a common mobilization process associated with acquisition of the blaVIM-2 and blaVIM-1 genes.

KW - Pseudomonas aeruginosa

KW - Carbapenemase

KW - Plasmid-mediated resistance

KW - Transposon

U2 - 10.1128/aac.02532-16

DO - 10.1128/aac.02532-16

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C2 - 28193652

SN - 0066-4804

VL - 61

SP - 1

EP - 6

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 5

M1 - e02532-16

ER -

Characterization of the pJB12 Plasmid from Pseudomonas aeruginosa reveals Tn6352, a novel putative transposon associated with mobilization of the blaVIM-2-harboring In58 integron

Abstract

Keywords

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