Chitosan-based nanoparticles for rosmarinic acid ocular delivery-In vitro tests

Sara Baptista da Silva; Domingos Ferreira; Manuela Pintado; Bruno Sarmento

doi:10.1016/j.ijbiomac.2015.11.070

Chitosan-based nanoparticles for rosmarinic acid ocular delivery-In vitro tests

Sara Baptista da Silva^*, Domingos Ferreira, Manuela Pintado, Bruno Sarmento

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

129 Citations (Scopus)

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Abstract

In this study, chitosan nanoparticles were used to encapsulate antioxidant rosmarinic acid, Salvia officinalis (sage) and Satureja montana (savory) extracts as rosmarinic acid natural vehicles. The nanoparticles were prepared by ionic gelation using chitosan and sodium tripolyphosphate (TPP) in a mass ratio of 7:1, at pH 5.8. Particle size distribution analysis and transmission electron microscopy (TEM) confirmed the size ranging from 200 to 300nm, while surface charge of nanoparticles ranged from 20 to 30mV. Nanoparticles demonstrate to be safe without relevant cytotoxicity against retina pigment epithelium (ARPE-19) and human cornea cell line (HCE-T). The permeability study in HCE monolayer cell line showed an apparent permeability coefficient Papp of 3.41±0.99×10-5 and 3.24±0.79×10-5cm/s for rosmarinic acid loaded chitosan nanoparticles and free in solution, respectively. In ARPE-19 monolayer cell line the Papp was 3.39±0.18×10-5 and 3.60±0.05×10-5cm/s for rosmarinic acid loaded chitosan nanoparticles and free in solution, respectively. Considering the mucin interaction method, nanoparticles indicate mucoadhesive proprieties suggesting an increased retention time over the ocular mucosa after instillation. These nanoparticles may be promising drug delivery systems for ocular application in oxidative eye conditions.

Original language	English
Pages (from-to)	112-120
Number of pages	9
Journal	International Journal of Biological Macromolecules
Volume	84
DOIs	https://doi.org/10.1016/j.ijbiomac.2015.11.070
Publication status	Published - 1 Mar 2016

Keywords

Cell models
Chitosan
Nanoparticles
Rosmarinic acid

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10.1016/j.ijbiomac.2015.11.070

19738046Accepted author manuscript, 724 KBLicence: CC BY-NC-ND

http://hdl.handle.net/10400.14/23071Licence: CC BY-NC-ND

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@article{28dcd751ec394c74a496127b516f3fd9,

title = "Chitosan-based nanoparticles for rosmarinic acid ocular delivery-In vitro tests",

abstract = "In this study, chitosan nanoparticles were used to encapsulate antioxidant rosmarinic acid, Salvia officinalis (sage) and Satureja montana (savory) extracts as rosmarinic acid natural vehicles. The nanoparticles were prepared by ionic gelation using chitosan and sodium tripolyphosphate (TPP) in a mass ratio of 7:1, at pH 5.8. Particle size distribution analysis and transmission electron microscopy (TEM) confirmed the size ranging from 200 to 300nm, while surface charge of nanoparticles ranged from 20 to 30mV. Nanoparticles demonstrate to be safe without relevant cytotoxicity against retina pigment epithelium (ARPE-19) and human cornea cell line (HCE-T). The permeability study in HCE monolayer cell line showed an apparent permeability coefficient Papp of 3.41±0.99×10-5 and 3.24±0.79×10-5cm/s for rosmarinic acid loaded chitosan nanoparticles and free in solution, respectively. In ARPE-19 monolayer cell line the Papp was 3.39±0.18×10-5 and 3.60±0.05×10-5cm/s for rosmarinic acid loaded chitosan nanoparticles and free in solution, respectively. Considering the mucin interaction method, nanoparticles indicate mucoadhesive proprieties suggesting an increased retention time over the ocular mucosa after instillation. These nanoparticles may be promising drug delivery systems for ocular application in oxidative eye conditions.",

keywords = "Cell models, Chitosan, Nanoparticles, Rosmarinic acid",

author = "Silva, \{Sara Baptista da\} and Domingos Ferreira and Manuela Pintado and Bruno Sarmento",

note = "Funding Information: This work was also financed by European Regional Development Fund (ERDF) through the Programa Operacional Factores de Competitividade—COMPETE, by Portuguese funds through FCT—Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia in the framework of the project PEst-OE/EQB/LA0016/2013, and co-financed by North Portugal Regional Operational Programme (ON.2–O Novo Norte) in the framework of project SAESCTN-PIIC\&DT/2011, under the National Strategic Reference Framework (NSRF). Funding Information: Funding for author Sara B. Silva was via a PhD fellowship, administered by Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia ( SFRH/BD/61423/2009 ). This work was supported by National Funds from FCT—Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia through project PEst-OE/EQB/LA0016/2013. Publisher Copyright: {\textcopyright} 2015 Elsevier B.V.",

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AU - Silva, Sara Baptista da

AU - Ferreira, Domingos

AU - Pintado, Manuela

AU - Sarmento, Bruno

N1 - Funding Information: This work was also financed by European Regional Development Fund (ERDF) through the Programa Operacional Factores de Competitividade—COMPETE, by Portuguese funds through FCT—Fundação para a Ciência e a Tecnologia in the framework of the project PEst-OE/EQB/LA0016/2013, and co-financed by North Portugal Regional Operational Programme (ON.2–O Novo Norte) in the framework of project SAESCTN-PIIC&DT/2011, under the National Strategic Reference Framework (NSRF). Funding Information: Funding for author Sara B. Silva was via a PhD fellowship, administered by Fundação para a Ciência e a Tecnologia ( SFRH/BD/61423/2009 ). This work was supported by National Funds from FCT—Fundação para a Ciência e a Tecnologia through project PEst-OE/EQB/LA0016/2013. Publisher Copyright: © 2015 Elsevier B.V.

PY - 2016/3/1

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N2 - In this study, chitosan nanoparticles were used to encapsulate antioxidant rosmarinic acid, Salvia officinalis (sage) and Satureja montana (savory) extracts as rosmarinic acid natural vehicles. The nanoparticles were prepared by ionic gelation using chitosan and sodium tripolyphosphate (TPP) in a mass ratio of 7:1, at pH 5.8. Particle size distribution analysis and transmission electron microscopy (TEM) confirmed the size ranging from 200 to 300nm, while surface charge of nanoparticles ranged from 20 to 30mV. Nanoparticles demonstrate to be safe without relevant cytotoxicity against retina pigment epithelium (ARPE-19) and human cornea cell line (HCE-T). The permeability study in HCE monolayer cell line showed an apparent permeability coefficient Papp of 3.41±0.99×10-5 and 3.24±0.79×10-5cm/s for rosmarinic acid loaded chitosan nanoparticles and free in solution, respectively. In ARPE-19 monolayer cell line the Papp was 3.39±0.18×10-5 and 3.60±0.05×10-5cm/s for rosmarinic acid loaded chitosan nanoparticles and free in solution, respectively. Considering the mucin interaction method, nanoparticles indicate mucoadhesive proprieties suggesting an increased retention time over the ocular mucosa after instillation. These nanoparticles may be promising drug delivery systems for ocular application in oxidative eye conditions.

AB - In this study, chitosan nanoparticles were used to encapsulate antioxidant rosmarinic acid, Salvia officinalis (sage) and Satureja montana (savory) extracts as rosmarinic acid natural vehicles. The nanoparticles were prepared by ionic gelation using chitosan and sodium tripolyphosphate (TPP) in a mass ratio of 7:1, at pH 5.8. Particle size distribution analysis and transmission electron microscopy (TEM) confirmed the size ranging from 200 to 300nm, while surface charge of nanoparticles ranged from 20 to 30mV. Nanoparticles demonstrate to be safe without relevant cytotoxicity against retina pigment epithelium (ARPE-19) and human cornea cell line (HCE-T). The permeability study in HCE monolayer cell line showed an apparent permeability coefficient Papp of 3.41±0.99×10-5 and 3.24±0.79×10-5cm/s for rosmarinic acid loaded chitosan nanoparticles and free in solution, respectively. In ARPE-19 monolayer cell line the Papp was 3.39±0.18×10-5 and 3.60±0.05×10-5cm/s for rosmarinic acid loaded chitosan nanoparticles and free in solution, respectively. Considering the mucin interaction method, nanoparticles indicate mucoadhesive proprieties suggesting an increased retention time over the ocular mucosa after instillation. These nanoparticles may be promising drug delivery systems for ocular application in oxidative eye conditions.

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JO - International Journal of Biological Macromolecules

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ER -

Chitosan-based nanoparticles for rosmarinic acid ocular delivery-In vitro tests

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Keywords

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