Chitosan nanoparticles as alternative anti-staphylococci agents: bactericidal, antibiofilm and antiadhesive effects

E. M. Costa, S. Silva, S. Vicente, C. Neto, P. M. Castro, M. Veiga, R. Madureira, F. Tavaria, M. M. Pintado*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

63 Citations (Scopus)

Abstract

Chitosan is a biocompatible, bioactive, non-toxic polymer that due to these characteristics has been widely used as a carrier for targeted delivery of bioactive molecules. In recent years, and considering that chitosan has a strong antimicrobial potential, the scientific community's focus has shifted onto the possible antimicrobial activity of chitosan nanoparticles. With this in mind, the aim of this work was to produce low molecular weight chitosan nanoparticles, through the ionic gelation method and characterize their potential biological activity against three staphylococci (MSSA, MRSA and MRSE) in planktonic and sessile environments. The chitosan nanoparticles produced had an average size of 244 ± 12 nm, an average charge of 17.3 ± 1.4 mV and had a MIC of 1.25 mg/mL for all tested microorganisms. Bactericidal activity was only registered for MSSA and MRSA with the time-inhibition curves showing bactericidal activity within 1 h. Assays regarding chitosan nanoparticles’ impact upon sessile populations showed that they were effective in preventing MRSE adhesion and highly effective in reducing MRSA and MSSA biofilm formation.
Original languageEnglish
Pages (from-to)221-226
Number of pages6
JournalMaterials Science and Engineering C
Volume79
DOIs
Publication statusPublished - 1 Oct 2017

Keywords

  • Antiadhesive
  • Antibiofilm
  • Antimicrobial
  • Chitosan nanoparticles
  • MRSA
  • MRSE

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