TY - JOUR
T1 - Chondrogenesis-inductive nanofibrous substrate using both biological fluids and mesenchymal stem cells from an autologous source
AU - Casanova, Marta R.
AU - Silva, Marta Alves da
AU - Costa-Pinto, Ana R.
AU - Reis, Rui L.
AU - Martins, Albino
AU - Neves, Nuno M.
N1 - Funding Information:
The authors would like to acknowledge the Portuguese Foundation for Science and Technology (FCT) for the PhD grant of MRC ( PD/BD/113797/2015 ) financed by the FCT Doctoral Program on Advanced Therapies for Health (PATH) ( FSE/POCH/PD/169/2013 ), the Post-doc fellowships of MAS and ARP (SFRH/BPD/73322/2010 and SFRH/BPD/90332/2012), the IF grant of AM ( IF/00376/2014 ), and the projects SPARTAN ( PTDC/CTM-BIO/4388/2014 ) and FRONthera ( NORTE-01-0145-FEDER-0000232 ).
Funding Information:
The authors would like to acknowledge the Portuguese Foundation for Science and Technology (FCT) for the PhD grant of MRC (PD/BD/113797/2015) financed by the FCT Doctoral Program on Advanced Therapies for Health (PATH) (FSE/POCH/PD/169/2013), the Post-doc fellowships of MAS and ARP (SFRH/BPD/73322/2010 and SFRH/BPD/90332/2012), the IF grant of AM (IF/00376/2014), and the projects SPARTAN (PTDC/CTM-BIO/4388/2014) and FRONthera (NORTE-01-0145-FEDER-0000232).
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/5
Y1 - 2019/5
N2 - During the last decade, many cartilage tissue engineering strategies have been developed, being the stem cell-based approach one of the most promising. Transforming Growth Factor-β3 (TGF-β3) and Insulin-like Growth Factor-I (IGF-I) are key proteins involved in the regulation of chondrogenic differentiation. Therefore, these two growth factors (GFs) were immobilized at the surface of a single electrospun nanofibrous mesh (NFM) aiming to differentiate human Bone Marrow-derived Mesenchymal Stem Cells (hBM-MSCs). The immobilization of defined antibodies (i.e. anti-TGF-β3 and anti-IGF-I) allows the selective retrieval of the abovementioned GFs from human platelet lysates (PL). Biochemical assays, involving hBM-MSCs cultured on biofunctional nanofibrous substrates under basal culture medium during 28 days, confirm the biological activity of bound TGF-β3 and IGF-I. Specifically, the typical spherical morphology of chondrocytes and the immunolocalization of collagen type II confirmed the formation of a cartilaginous ECM. Therefore, the proposed biofunctional nanofibrous substrate is able to promote chondrogenesis.
AB - During the last decade, many cartilage tissue engineering strategies have been developed, being the stem cell-based approach one of the most promising. Transforming Growth Factor-β3 (TGF-β3) and Insulin-like Growth Factor-I (IGF-I) are key proteins involved in the regulation of chondrogenic differentiation. Therefore, these two growth factors (GFs) were immobilized at the surface of a single electrospun nanofibrous mesh (NFM) aiming to differentiate human Bone Marrow-derived Mesenchymal Stem Cells (hBM-MSCs). The immobilization of defined antibodies (i.e. anti-TGF-β3 and anti-IGF-I) allows the selective retrieval of the abovementioned GFs from human platelet lysates (PL). Biochemical assays, involving hBM-MSCs cultured on biofunctional nanofibrous substrates under basal culture medium during 28 days, confirm the biological activity of bound TGF-β3 and IGF-I. Specifically, the typical spherical morphology of chondrocytes and the immunolocalization of collagen type II confirmed the formation of a cartilaginous ECM. Therefore, the proposed biofunctional nanofibrous substrate is able to promote chondrogenesis.
KW - Chondrogenic differentiation
KW - Electrospun nanofibrous meshes
KW - Insulin-like growth factor-I (IGF-I)
KW - Platelet lysates
KW - Transforming growth factor-β3 (TGF-β3)
UR - http://www.scopus.com/inward/record.url?scp=85060200299&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2019.01.069
DO - 10.1016/j.msec.2019.01.069
M3 - Article
C2 - 30813000
AN - SCOPUS:85060200299
SN - 0928-4931
VL - 98
SP - 1169
EP - 1178
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
ER -