TY - JOUR
T1 - Cinnamaldehyde induces changes in the protein profile of Salmonella Typhimurium biofilm
AU - Silva, Alex Fiori
AU - Santos, Adriele Rodrigues dos
AU - Trevisan, Daliah Alves Coelho
AU - Ribeiro, Alessandra Braga
AU - Zanetti Campanerut-Sá, Paula Aline
AU - Kukolj, Caroline
AU - Souza, Emanuel Maltempi de
AU - Cardoso, Rosilene Fressatti
AU - Svidzinski, Terezinha Inez Estivalet
AU - Filho, Benicio Alves de Abreu
AU - Junior, Miguel Machinski
AU - Mikcha, Jane Martha Graton
N1 - Funding Information:
The authors thank the Coordenação de Aperfeiçoamento de Pessoal de Nível superior (CAPES) and the Complexo de Central de Apoio a Pesquisa (COMCAP) of the State University of Maringá.
Publisher Copyright:
© 2017 Institut Pasteur
PY - 2018/1
Y1 - 2018/1
N2 - The effect of cinnamaldehyde against biofilm cells of Salmonella Typhimurium ATCC 14028 was evaluated. We also assessed differential protein patterns that were expressed by biofilms compared with planktonic cells and protein expression by cinnamaldehyde-treated biofilms cells. This compound decreased biofilm biomass and metabolic activity of biofilms at both concentrations tested. Cinnamaldehyde treatment reduced the number of attached cells in polypropylene, reflected by colony count and scanning electron microscopy. The proteomic analysis of biofilms compared with planktonic cells indicated that several proteins were upregulated or downregulated, especially proteins that are involved in energy metabolism. Peroxiredoxin, ATP synthase alpha chain protein, conjugal transfer nickase/helicase TraI and elongation factor G were upregulated in untreated-biofilm cells, and their expression decreased as a function of cinnamaldehyde treatment. Cinnamaldehyde had antibiofilm activity, and several differentially expressed proteins identified provide potential and interesting targets to explore new control strategies for S. Typhimurium biofilms.
AB - The effect of cinnamaldehyde against biofilm cells of Salmonella Typhimurium ATCC 14028 was evaluated. We also assessed differential protein patterns that were expressed by biofilms compared with planktonic cells and protein expression by cinnamaldehyde-treated biofilms cells. This compound decreased biofilm biomass and metabolic activity of biofilms at both concentrations tested. Cinnamaldehyde treatment reduced the number of attached cells in polypropylene, reflected by colony count and scanning electron microscopy. The proteomic analysis of biofilms compared with planktonic cells indicated that several proteins were upregulated or downregulated, especially proteins that are involved in energy metabolism. Peroxiredoxin, ATP synthase alpha chain protein, conjugal transfer nickase/helicase TraI and elongation factor G were upregulated in untreated-biofilm cells, and their expression decreased as a function of cinnamaldehyde treatment. Cinnamaldehyde had antibiofilm activity, and several differentially expressed proteins identified provide potential and interesting targets to explore new control strategies for S. Typhimurium biofilms.
KW - Biofilm
KW - Cinnamaldehyde
KW - Polypropylene
KW - Proteomics
KW - Salmonella Typhimurium
UR - https://www.scopus.com/pages/publications/85032211301
U2 - 10.1016/j.resmic.2017.09.007
DO - 10.1016/j.resmic.2017.09.007
M3 - Article
C2 - 28974445
AN - SCOPUS:85032211301
SN - 0923-2508
VL - 169
SP - 33
EP - 43
JO - Research in Microbiology
JF - Research in Microbiology
IS - 1
ER -