@article{3b36d328968f47cc954cee47a2f20b49,
title = "Co-transplantation of adipose tissue-derived stromal cells and olfactory ensheathing cells for spinal cord injury repair",
abstract = "Patients suffering from spinal cord injury (SCI) still have a dismal prognosis. Despite all the efforts developed in this area, currently there are no effective treatments. Therefore, cell therapies have been proposed as a viable alternative to the current treatments used. Adipose tissue-derived stromal cells (ASCs) and olfactory ensheathing cells (OECs) have been used with promising results in different models of SCI, namely due to the regenerative properties of the secretome of the first, and the guidance capability of the second. Using an in vitro model of axonal growth, the dorsal root ganglia explants, we demonstrated that OECs induce neurite outgrowth mainly through cell-cell interactions, while ASCs' effects are strongly mediated by the release of paracrine factors. A proteomic analysis of ASCs' secretome revealed the presence of proteins involved in VEGF, PI3K, and Cadherin signaling pathways, which may be responsible for the effects observed. Then, the cotransplantation of ASCs and OECs showed to improve motor deficits of SCI-rats. Particular parameters of movement such as stepping, coordination, and toe clearance were improved in rats that received the transplant of cells, in comparison to nontreated rats. A histological analysis of the spinal cord tissues revealed that transplantation of ASCs and OECs had a major effect on the reduction of inflammatory cells close the lesion site. A slight reduction of astrogliosis was also evident. Overall, the results obtained with the present work indicate that the cotransplantation of ASCs and OECs brings important functional benefits to the injured spinal cord. Stem Cells 2018;36:696–708.",
keywords = "Adipose tissue-derived stem cells, Axonal regeneration, Olfactory ensheathing cells, Regenerative medicine, Spinal cord injury",
author = "Gomes, {Eduardo D.} and Mendes, {Sofia S.} and Assun{\c c}{\~a}o-Silva, {Rita C.} and Teixeira, {F{\'a}bio G.} and Pires, {Ana O.} and Anjo, {Sandra I.} and Bruno Manadas and Hugo Leite-Almeida and Gimble, {Jeffrey M.} and Nuno Sousa and Lepore, {Angelo C.} and Silva, {Nuno A.} and Salgado, {Ant{\'o}nio J.}",
note = "Funding Information: We acknowledge the financial support from Pr{\'e}mios Santa Casa Neuroci{\^e}ncias—Prize Melo e Castro for Spinal Cord Injury Research; from Portuguese Foundation for Science and Technology [Doctoral fellowship (SFRH/BD/103075/2014) to E.D.G; Doctoral fellowship (PDE/BDE/113596/2015) to R.C.A-S.; Post-Doctoral fellowship (SFRH/BPD/118408/2016) to F.G.T.; Doctoral fellowship (SFRH/BD/81495/2011) to S.I.A.; Post-Doctoral fellowship (SFRH/BPD/97701/2013) to N.A.S.; IF Development Grant to A.J.S.]. Partial supported by “Projeto 3599-Promover a Produc¸{\~a}o Cient{\'i}fica e Desenvolvimento Tec-nol{\'o}gico e a Constituic¸{\~a}o de Redes Tem{\'a}ticas (3599-PPCDT),” reference PTDC/DTP-FTO/5109/2014. This article is a result of the project (NORTE-01-0145-FEDER-000013), supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF); Cofinanciado pelo Programa Operacional Regional do Norte (ON.2 SR&TD Integrated Program – NORTE-07-0124-FEDER-000021), ao abrigo do Quadro de Refer{\^e}ncia Estrat{\'e}gico Nacional (QREN), atrav{\'e}s do Fundo Europeu de Desenvolvi-mento Regional (FEDER); Projeto Estrat{\'e}gico – LA 26 – 2011– 2012 and Projeto Estrat{\'e}gico – LA 26 – 2013-2014 cofinan-ciado por fundos nacionais, atrav{\'e}s da Fundac¸{\~a}o para a Ci{\^e}n-cia e a Tecnologia (PEst-C/SAU/LA0026/2011; PEst-C/SAU/ LA0026/2013), e pelo Fundo Europeu de Desenvolvimento Regional (FEDER), atrav{\'e}s do COMPETE (FCOMP-01-0124-FEDER-022724; FCOMP-01-0124-FEDER-037298). Support also from PTDC/NEU-SCC/7051/2014; POCI-01-0145-FEDER-007440; PTDC/NEU-NMC/0205/2012; UID/NEU/04539/2013; The National Mass Spectrometry Network (RNEM) (REDE/1506/ REM/2005). This work has been funded by FEDER funds, through the Competitiveness Factors Operational Programme (COMPETE), and by National funds, through the Foundation for Science and Technology (FCT), under the scope of the project POCI-01-0145-FEDER-007038. We also want to acknowledge Rui Lima, Nadiya Kubasova, and Deolinda Santinha for the help with DRG experiments and analysis; Eduarda Oliveira and Nat{\'a}lia Vasconcelos for assistance in the in vivo experiments. Publisher Copyright: {\textcopyright} AlphaMed Press 2018",
year = "2018",
month = may,
doi = "10.1002/stem.2785",
language = "English",
volume = "36",
pages = "696--708",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "Oxford University Press",
number = "5",
}