Collagen-based bioactive bromelain hydrolysate from salt-cured cod skin

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Considerable amounts of fish processing by-products are discarded each year. About 30% of this material may be skin and bone. Fish skin has more than 80% of its total protein content as collagen. Furthermore, in recent years, there has been a growing demand for collagen-based peptides due to their beneficial health effects. So, the objective of the present study was to optimise the obtaining bioactive hydrolysates from salt-cured cod skin using the protease Bromelain at 0.5% (w/w) concentration. This study developed a sustainable process that consumes less time and energy and uses an alternative source as raw material. In addition, bromelain allows hydrolysates with important antioxidant (ORAC, 514 μmol Trolox Equivalent/g protein) and antihypertensive activities (inhibition of ACE, IC50 of 166 μg protein/mL) as well as excellent biocompatibility with dermal and subcutaneous cells.
Original languageEnglish
Article number8538
Number of pages12
JournalApplied Sciences
Issue number18
Publication statusPublished - 14 Sept 2021


  • Antihypertensive
  • Antioxidant
  • Atlantic cod skin
  • Bioactive peptides
  • Bromelain
  • Collagen
  • Enzymatic hydrolysis
  • Fishery by-products


Dive into the research topics of 'Collagen-based bioactive bromelain hydrolysate from salt-cured cod skin'. Together they form a unique fingerprint.

Cite this