TY - JOUR
T1 - Comparison of ubiquitous antibiotic-resistant Enterobacteriaceae populations isolated from wastewaters, surface waters and drinking waters
AU - Figueira, Vânia
AU - Serra, Elizabete A.
AU - Vaz-Moreira, Ivone
AU - Brandão, Teresa R. S.
AU - Manaia, Célia M.
PY - 2012/3/5
Y1 - 2012/3/5
N2 - This study aimed at assessing the role of ubiquitous (non-Escherichia coli) Enterobacteriaceae in the dissemination of antimicrobial resistance through the urban water cycle. Enterobacteriaceae isolated from a municipal wastewater treatment plant (111 isolates), urban water streams (33 isolates) and drinking water (123 isolates) were compared in terms of: (i) genera distribution, (ii) resistance to 12 antibiotics, and (iii) class 1 and class 2 integrons. The predominant bacterial genera were the same in the different types of water, although with a distinct pattern of species. The most prevalent resistance phenotypes were observed for amoxicillin, ticarcillin, cephalothin and sulphamethoxazole (24-59% in the three types of water). No resistance against ceftazidime or meropenem was observed. Resistance to cephalothin, amoxicillin and sulphamethoxazole was significantly more prevalent in drinking water, water streams and wastewater, respectively, than in the other types of water. It was possible to recognize antibiotic-resistance associations, namely for the pairs streptomycin-tetracycline (positive) and ticarcillin-cephalotin (negative). Class 1 and/or class 2 integrons with similar gene cassettes were detected in the three types of water. This study demonstrated that Enterobacteriaceae are important vehicles of antibiotic resistance, namely in drinking water.
AB - This study aimed at assessing the role of ubiquitous (non-Escherichia coli) Enterobacteriaceae in the dissemination of antimicrobial resistance through the urban water cycle. Enterobacteriaceae isolated from a municipal wastewater treatment plant (111 isolates), urban water streams (33 isolates) and drinking water (123 isolates) were compared in terms of: (i) genera distribution, (ii) resistance to 12 antibiotics, and (iii) class 1 and class 2 integrons. The predominant bacterial genera were the same in the different types of water, although with a distinct pattern of species. The most prevalent resistance phenotypes were observed for amoxicillin, ticarcillin, cephalothin and sulphamethoxazole (24-59% in the three types of water). No resistance against ceftazidime or meropenem was observed. Resistance to cephalothin, amoxicillin and sulphamethoxazole was significantly more prevalent in drinking water, water streams and wastewater, respectively, than in the other types of water. It was possible to recognize antibiotic-resistance associations, namely for the pairs streptomycin-tetracycline (positive) and ticarcillin-cephalotin (negative). Class 1 and/or class 2 integrons with similar gene cassettes were detected in the three types of water. This study demonstrated that Enterobacteriaceae are important vehicles of antibiotic resistance, namely in drinking water.
KW - Antibiotic resistance
KW - Class 1 integron
KW - Class 2 integron
KW - Drinking water
KW - Fecal contamination
KW - Ubiquitous enterobacteriaceae
UR - http://www.scopus.com/inward/record.url?scp=84857510060&partnerID=8YFLogxK
U2 - 10.2166/wh.2011.002
DO - 10.2166/wh.2011.002
M3 - Article
C2 - 22361697
AN - SCOPUS:84857510060
VL - 10
SP - 1
EP - 10
JO - Journal of Water and Health
JF - Journal of Water and Health
SN - 1477-8920
IS - 1
ER -