Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: in vitro mineralization and biological evaluation

Filipa Castro, Viviana P. Ribeiro, António Ferreira, Ana L. Oliveira, Rui L. Reis, José A. Teixeira, Fernando Rocha

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Abstract

This work reports the biological evaluation of nanosized hydroxyapatite (HAp) previously synthesized by continuous-flow precipitation in a scaled-up meso oscillatory flow reactor (meso-OFR). Physicochemical characterization of the synthesized HApsuggests high surface reactivity namely because of its high specific surface area and low crystallinity. On the other hand, in vitro biomineralization assays demonstrated the apatite-forming activity of the prepared HAp and their higher surface reactivity when compared to a commercial HAp. Furthermore, human osteoblastic-like (Saos-2) cells culture evidenced that the synthesized HAp stimulated cell proliferation, especially when applied at lower concentrations (30 and 50 μgml-1), although its cellular uptake behavior. Therefore, the prepared HApshows immense potential as biomedical material, as well as drug and gene delivery vehicle. The results are also very promising regarding further scaling up of the process, as the designed methodology allow for the preparation in a continuous mode of nanosized HAp with controlled physico-chemical properties.
Original languageEnglish
Article number075404
Number of pages13
JournalMaterials Research Express
Volume3
Issue number7
DOIs
Publication statusPublished - Jul 2016

Keywords

  • Calcium phosphates
  • Mineralization
  • Nanohydroxyapatite
  • Oscillatory flow reactor
  • SBF

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