TY - JOUR
T1 - Continuous production of highly tuned silk/calcium-based composites
T2 - exploring new pathways for skin regeneration
AU - Veiga, Anabela
AU - Magalhães, Rui
AU - Duarte, Marta M.
AU - Dias, Juliana R.
AU - Alves, Nuno M.
AU - Costa-Pinto, Ana Rita
AU - Castro, Filipa
AU - Rocha, Fernando
AU - Oliveira, Ana L.
N1 - Funding Information:
Funding: This work was financially supported by: national funds through FCT (Foundation for Science and Technology) under the project UIDB/50016/2020 of the Centre for Biotechnology and Fine Chemistry-CBQF. This work was financially supported by LA/P/0045/2020 (ALiCE), UIDB/00511/2020, and UIDP/00511/2020 (LEPABE), funded by national funds through FCT/MCTES (PIDDAC); and FCT through the following Projects: UIDB/04044/2020, UIDP/04044/2020. This study was also supported by PAMI-ROTEIRO/0328/2013 (Nº 022158), MATIS (CENTRO-01-0145-FEDER-000014-3362) and Bone2Move (POCI-01-0145-FEDER-31146). A. Veiga gratefully acknowledges doctoral scholarship [2020.08683.BD] from FCT.
Funding Information:
This work was financially supported by: national funds through FCT (Foundation for Science and Technology) under the project UIDB/50016/2020 of the Centre for Biotechnology and Fine Chemistry-CBQF. This work was financially supported by LA/P/0045/2020 (AL-iCE), UIDB/00511/2020, and UIDP/00511/2020 (LEPABE), funded by national funds through FCT/MCTES (PIDDAC); and FCT through the following Projects: UIDB/04044/2020, UIDP/04044/2020. This study was also supported by PAMI-ROTEIRO/0328/2013 (N? 022158), MATIS (CENTRO-01-0145-FEDER-000014-3362) and Bone2Move (POCI-01-0145-FEDER-31146). A. Veiga gratefully acknowledges doctoral scholarship [2020.08683.BD] from FCT.
Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/4/1
Y1 - 2022/4/1
N2 - Calcium plays an important role in barrier function repair and skin homeostasis. In particular, calcium phosphates (CaPs) are well established materials for biomedical engineering due to their biocompatibility. To generate biomaterials with a more complete set of biological properties, previously discarded silk sericin (SS) has been recovered and used as a template to grow CaPs. Crucial characteristics for skin applications, such as antibacterial activity, can be further enhanced by doping CaPs with cerium (Ce) ions. The effectiveness of cell attachment and growth on the materials highly depends on their morphology, particle size distribution, and chemical composition. These characteristics can be tailored through the application of oscillatory flow technology, which provides precise mixing control of the reaction medium. Thus, in the present work, CaP/SS and CaP/SS/Ce particles were fabricated for the first time using a modular oscillatory flow plate reactor (MOFPR) in a continuous mode. Furthermore, the biological behavior of both these composites and of previously produced pure CaPs was assessed using human dermal fibroblasts (HDFs). It was demonstrated that both CaP based with plate-shaped nanoparticles and CaP-SS-based composites significantly improved cell viability and proliferation over time. The results obtained represent a first step towards the reinvention of CaPs for skin engineering.
AB - Calcium plays an important role in barrier function repair and skin homeostasis. In particular, calcium phosphates (CaPs) are well established materials for biomedical engineering due to their biocompatibility. To generate biomaterials with a more complete set of biological properties, previously discarded silk sericin (SS) has been recovered and used as a template to grow CaPs. Crucial characteristics for skin applications, such as antibacterial activity, can be further enhanced by doping CaPs with cerium (Ce) ions. The effectiveness of cell attachment and growth on the materials highly depends on their morphology, particle size distribution, and chemical composition. These characteristics can be tailored through the application of oscillatory flow technology, which provides precise mixing control of the reaction medium. Thus, in the present work, CaP/SS and CaP/SS/Ce particles were fabricated for the first time using a modular oscillatory flow plate reactor (MOFPR) in a continuous mode. Furthermore, the biological behavior of both these composites and of previously produced pure CaPs was assessed using human dermal fibroblasts (HDFs). It was demonstrated that both CaP based with plate-shaped nanoparticles and CaP-SS-based composites significantly improved cell viability and proliferation over time. The results obtained represent a first step towards the reinvention of CaPs for skin engineering.
KW - Calcium phosphate-based materials (CaP)
KW - Cerium (Ce)
KW - Human dermal fibroblasts (HDFs)
KW - Modular oscillatory flow plate reactor (MOPR)
KW - Silk sericin (SS)
KW - Skin regeneration
UR - http://www.scopus.com/inward/record.url?scp=85128098694&partnerID=8YFLogxK
U2 - 10.3390/molecules27072249
DO - 10.3390/molecules27072249
M3 - Article
C2 - 35408647
AN - SCOPUS:85128098694
SN - 1420-3049
VL - 27
JO - Molecules
JF - Molecules
IS - 7
M1 - 2249
ER -